Abstract Background Local sequence context is known to have an impact on the mutational pattern seen in cancer. The RAS genes and a smoking carcinogen, Benzo[a]pyrene diol epoxide (BPDE), been utilised explore these effects. BPDE form adduct at guanines number of gene sites, KRAS codons 12, 13 14, NRAS codon HRAS 12 14. Results Molecular modelling techniques, along with multivariate analysis, d...

A molecular biological rationale for the linear nonthreshold dose-response pattern for carcinogenesis is presented based on the mutagenic activation of oncogens as the basis of initiation. The approach assumes that the linear nonthreshold dose pattern at very low doses applies only to tissues that are promoted by intrinsic and extrinsic agents other than the one being modeled, and that risk is ...

A major focus of our research is the characterization and quantitation of carcinogen-protein adducts. This was done in the past primarily by electron-capture NICI-GC-MS of derivatized adduct moieties following their hydrolytic release from the proteins. The actual analyses were thus carried out on small molecules identical to or structurally similar to the original carcinogen. Most experiments,...