MicroRNAs (miRNAs) have been found to be stable in the circulation of cancer patients raising their potential as circulating biomarkers of disease. The specific source and role, however, of miRNAs in the circulation is unknown and requires elucidation to determine their true potential. In this study, along with primary tissue explants and primary stromal cells, three breast cancer cell lines we...

We have developed a paracrine signaling assay capable of mimicking inductive events in the early vertebrate embryo. RNA encoding one or more secreted proteins is microinjected into a Xenopus laevis oocyte. After a brief incubation to allow translation, a piece of embryonic tissue competent to respond to the signaling protein is grafted onto the oocyte. The secreted protein's effect on the graft...

OBJECTIVE Visceral adipose tissue (VAT) is more closely linked to insulin resistance than subcutaneous adipose tissue (SAT). We conducted a quantitative analysis of the secretomes of VAT and SAT to identify differences in adipokine secretion that account for the adverse metabolic consequences of VAT. RESEARCH DESIGN AND METHODS We used lectin affinity chromatography followed by comparison of ...

PURPOSE Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for c...

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one these species, the region for traditional medicine ornamental purposes. Therefore, optimizing alternative micropropagation protocols tissue culture conditions secondary metabolite production needs attention. effect Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot r...

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct orga...

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct orga...

Pith tissue from Nicotiana tabacum L. cv ;Maryland Mammoth' or ;Wisconsin 38' was isolated, free of vascular tissue, and cultured on a medium containing auxin but no cytokinin. Explants from the apical 1 cm of stem, within the pith rib meristem, initiated callus growth with 100% efficiency. Macroscopically visible callus was evident 5 days after the tissue was isolated, and the cultures grew pe...