A subset of peripheral blood T lymphocytes coexpressing CD3 and IgG Fc receptors (FcR) (CD16/Leu-11 antigen) have been identified, isolated, and functionally characterized. The CD3+, CD16+ cells were established in short-term culture using growth medium containing interleukin 2 (IL-2). Both the freshly isolated cells and the cultured cell line stably expressed the CD3+, CD16+ phenotype. Further...

As HIV infected individuals live longer, the prevalence of HIV associated neurocognitive disorders is increasing, despite successful antiretroviral therapy. CD14(+)CD16(+) monocytes are critical to the neuropathogenesis of HIV as they promote viral seeding of the brain and establish neuroinflammation. The mechanisms by which HIV infected and uninfected monocytes cross the blood brain barrier an...

Much remains to be learned about the physiologic events that promote monocytes to become lymph-homing dendritic cells (DCs). In a model of transendothelial trafficking, some monocytes become DCs in response to endogenous signals. These DCs migrate across endothelium in the ablumenal-to-lumenal direction (reverse transmigration), reminiscent of the migration into lymphatic vessels. Here we show ...

Background Cellular immunity of Behçet’s Disease (BD) remains poorly understood. Previous work has provided clues pointing to most innate and adaptive immune cell types in BD, but strong signals from non-immunogenetic studies are rare often inconclusive. Objectives Here we aimed identify BD/HD discriminant single profiles semi-biased targeted approaches determine their significance at a BD rele...

__________________________________________________________________________________________ Monocytes are comprised of three phenotypically and functionally distinct subsets: classical CD14++CD16-, intermediate CD14++CD16+ and non-classical CD14+CD16++ cells that can play differential roles in regulation of both systemic and local inflammatory processes. In addition, these monocyte subsets repre...

Peripheral blood lymphocytes cultured in recombinant interleukin 2 during 3 to 5 days (short-term cultures) develop the ability to lyse natural killer-resistant tumor lines and fresh tumor cells, i.e., express lymphokine-activated killer (LAK) function. Phenotypic analysis has shown these cells to be natural killer cells, i.e., CD16+ and/or Leu 19+ cells. CD3+,CD16- T-cells, instead, develop ve...

BACKGROUND Activated platelets exert a pro-inflammatory action that can be largely ascribed to their ability to interact with leukocytes and modulate their activity. We hypothesized that platelet activation and consequent formation of monocyte-platelet aggregates (MPA) induces a pro-inflammatory phenotype in circulating monocytes. METHODOLOGY/PRINCIPAL FINDINGS CD62P(+) platelets and MPA were...

Background Macrophages strongly contribute to the pathogenesis of rheumatoid arthritis (RA), initiating inflammatory response, join damage, but also may promote resolution inflammation and restoration tissue immune-homeostasis [1,2]. This seems be related an unbalanced immunological response mediated by macrophages through their polarization into “classically” “alternatively” activated phenotyp...

Formation and deposition of immune complexes (ICs) are hallmarks various autoimmune diseases. Detection ICs by IC receptors on leukocytes induces downstream signaling shapes the local response. In many cases pathological relevance is not well understood. We here show that induce a distinct migratory response, i.e., haptokinesis in 6-sulfo LacNAc+ monocytes (slanMo) non-classical (ncMo) but inte...