background: vhl (von hippel-lindau), runx-3 (runt-related transcription factor 3), e-cadherin (epithelial cadherin), p15 (ink4a, cyclin dependent kinase inhibitor), and p16 (ink4b) genes are essential in hematopoiesis. the aim of this study was to explore the correlation between gene expression and promoter methylation in cd34+ stem cells before and after differentiation to erythroid lineage. m...

background: umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell transplantation (hsct), used in leukemia treatment. cd26+ cells, a fraction of cd34 positive cells, are a major population of ucb cells which negatively regulate the in vivo homing and engraftment of hscs. cd26 is highly expressed in various cells such as hscs, immune cells, fibroblasts, and epithelial ce...

OBJECTIVE Emerging evidence suggests that human blood contains bone marrow (BM)-derived endothelial progenitor cells that contribute to postnatal neovascularization. Clinical trials demonstrated that administration of BM-cells can enhance neovascularization. Most studies, however, used crude cell populations. Identifying the role of different cell populations is important for developing improve...

Four-color flow cytometry was used with a cocktail of antibodies to identify and isolate CD34+ hematopoietic progenitors from normal human peripheral blood (PB) and bone marrow (BM). Mature cells that did not contain colony forming cells were resolved from immature cells using antibodies for T lymphocytes (CD3), B lymphocytes (CD20), monocytes (CD14), and granulocytes (CD11b). Immature cells we...

We used a mouse transplantation model to address the recent controversy about CD34 expression by hematopoietic stem cells. Cells from Ly-5.1 C57BL/6 mice were used as donor cells and Ly-5.2 mice were the recipients. The test cells were transplanted together with compromised marrow cells of Ly-5.2 mice. First, we confirmed that the majority of the stem cells with long-term engraftment capabiliti...

All seven of a set of CD34 monoclonal antibodies that recognize epitopes on an approximately 110 Kd glycoprotein on human hemopoietic progenitor cells also bind to vascular endothelium. Capillaries of most tissues are CD34 positive, as are umbilical artery and, to a lesser extent, vein, but the endothelium of most large vessels and the endothelium of placental sinuses are not. Angioblastoma cel...

CD34+ hematopoietic stem cells are used clinically to support cytotoxic therapy, and recent studies raised hope that they could even serve as a cellular source for nonhematopoietic tissue engineering. Here, we examined in 18 volunteers the gene expressions of 1185 genes in highly enriched bone marrow CD34+ (BM-CD34+) or granulocyte-colony-stimulating factor-mobilized peripheral blood CD34+ (PB-...

We present cell cycling and functional evidence that the CD34+CD38- immunophenotype can be used to define a rare and primitive subpopulation of progenitor cells in umbilical cord blood. CD34+CD38- cells comprise 0.05% +/- 0.08% of the mononuclear cells present in cord blood. Cell cycle analysis with the fluorescent DNA stain 7-aminoactinomycin D showed that the percentage of CD34+ cells in cycl...

Circulating osteogenic precursor (COP) cells are a population of progenitor in the peripheral blood with capacity to form bone vitro and vivo . They have characteristics mesenchymal stem pool found marrow; however, more recently, COP has been identified markers hematopoietic lineage such as CD45 CD34. While this associated several pathologies, lack cell culture models inconsistent characterizat...

CD34 glycoprotein in human hematopoiesis is expressed on a subset of progenitor cells capable of self-renewal, multilineage differentiation, and hematopoietic reconstitution. Nucleolin is an abundant multifunctional phosphoprotein of growing eukaryotic cells, involved in regulation of gene transcription, chromatin remodeling, and RNA metabolism, whose transcripts are enriched in murine hematopo...