Bacterial attachment is a complex process affected by flow conditions, imparted stresses, and the surface properties structure of both supporting material cell. Experiments on initial cells bacterium Streptococcus gordonii (S. gordonii), an important early coloniser dental plaque, to samples stainless steel (SS) have been reported in this work. The primary aim motivating study was establish wha...

Cell adhesion is a multistep process involving initial contact of the cell with the surface, cell attachment and spreading on the surface, and cell growth (1.2). To determine whether cell adhesion is an active or passive phenomena, initial cell attachment assays have previously been carried out either at various temperatures (3) or in the presence of metabolic inhibitors (4). However, all of th...

Infection of cells by viruses begins with the attachment of the virus to the surface of the host cell (Dales, 1973; Lonberg-Holm & Philipson, 1974; Dimmock, 1982; Tardieu et al., 1982; Paulson, 1985; Marsh & Helenius, 1989). Attachment is mediated by the binding of a viral attachment protein (VAP) to a molecule on the cell surface acting as a virus receptor. The VAPs are the polypeptides formin...

Integration of living cells with novel microdevices requires the development of innovative technologies for manipulating cells. Chemical surface patterning has been proven as an effective method to control the attachment and growth of diverse cell populations. Patterning polyelectrolyte multilayers through the combination of layer-bylayer self-assembly technique and photolithography offers a si...

Cell attachment and neurite outgrowth by embryonic neural retinal cells were measured in separate quantitative assays to define differences in substrate preference and to demonstrate developmentally regulated changes in cellular response to different extracellular matrix glycoproteins. Cells attached to laminin, fibronectin, and collagen IV in a concentration-dependent fashion, though fibronect...

The laminin α2 chain is specifically expressed in the basement membrane surrounding muscle and nerve. We screened biologically active sequences in the mouse laminin N-terminal region of α2 chain using 216 soluble peptides and three recombinant proteins (rec-a2LN, rec-a2LN+, and rec-a2N) by both the peptide- or protein-coated plate and the peptide-conjugated Sepharose bead assays. Ten peptides s...

We explored the role that thrombospondin (TSP), a multifunctional extracellular matrix protein, plays in hematopoietic cell-cell and cell-matrix interactions. Thrombospondin synthesis is differentially regulated in human long-term bone marrow cultures. Consistent with this, human hematopoietic progenitor cells of all three lineages (erythrocyte, megakaryocyte, and granulocyte) use TSP as an att...

The kinetics of cell attachment and cell spreading on the coated surfaces of two classes of carbohydrate-reactive proteins, enzymes and lectins, have been compared with those on fibronectin-coated surfaces with the following results: (a) A remarkable similarity between the kinetics of cell attachment to fibronectin-coated and glycosidase-coated surfaces was found. In contrast, cell attachment k...

The aim of this study was to investigate whether peroxisome proliferator-activated receptor (PPAR)-gamma activation has an effect on the attachment of endometrial cells to peritoneal mesothelial cells in a well-established in vitro model of the early endometriotic lesion. The endometrial epithelial cell line EM42 and mesothelial cell line LP9 were used for this study. EM42 cells, LP9 cells or b...

The amino acid sequence RGD (arginine-glycine-aspartic acid) is highly conserved in the VP1 protein of foot-and-mouth disease virus (FMDV), despite being situated in the immunodominant hypervariable region between amino acids 135 and 160. RGD-containing proteins are known to be important in promoting cell attachment in several different systems, and we report here that synthetic peptides contai...