Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emer...

Even prior to observing the great plate anomaly, one of the most enduring questions in microbial ecology has been, ‘who is there, and what are they doing?’. While we are still far from getting a true handle on microbial diversity on this planet, the ‘who’ has become easier to address, with the rise of environmental surveys of 16S ribosomal RNA sequences in the 1990s, allowing charting of commun...

personalized medicine is significantly changing the fundamentals of healthcare. genetic diagnostic tests are used in various parts of medicine – from initial screening to the diagnosis and finally treatment. investigations in fields of drug development indicate that oncology may lead other therapeutic areas in the number of targeted therapies on the marketplace as well as in the pipeline, expec...

Single-cell sequencing technologies, i.e., single cell analysis followed by deep sequencing investigate cellular heterogeneity in many biological settings. It was only in the past year that single-cell sequencing analyses has been applied in the field of virology, providing new ways to explore viral diversity and cell response to viral infection, which are summarized in the present review.