Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET–based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET...

© 2001 European Molecular Biology Organization EMBO reports vol. 2 | no. 6 | 2001 459 butterfly, in Ontario. Canadian Food Inspection Agency, Plant Biotechnology Office. Trewavas, A.J. and Leaver, C.J. (2000) Conventional crops are the test of GM prejudice. Nature, 401, 640. USDA (2000) US Agricultural Outlook (July–August 2000).USDA–ERS.http://www.agribusiness.asn.au/ NewsLinksCongressEvents/A...

Aimed to achieve “waste control by waste” of coal-to-liquids (CTL) industry, coal gasification residue (CGR), the most typical CTL residue, and chemical wastewater (CCW) were used as aluminosilicate precursor auxiliary activator respectively prepare alkali activated materials (AAMs). CGR-based AAMs using a less concentrated NaOH solution than conventional, with CCW mixing water synthesized. The...

High doses of nitrogen (N) fertiliser input on permanent pastures are crucial in terms N surplus and losses. Quantitative analyses the response plant functional traits (PFT) driving crop growth rate (CGR) under low lacking frequently defoliated pastures. This study aimed to understand significance PFTs for productivity uptake grasslands by measuring dynamics tiller density (TD), weight (TW), le...