principle, lead to depletion of VIP in the airways and abnormalities characteristic of asthma. To examine whether catalytic antibodies can evolve over the normal course of antibody affinity maturation, we prepared monoclonal antibodies by immunization with a VIP-carrier protein conjugate. One of these antibodies hydrolyzed VIP with Km (Michaelis-Menten constant) 0.34 nM and turnover 8X10-4/min....

C57BL mice, upon immunization with chicken ),-globulin conjugated with (4hydroxy-3-nitrophenyl) acetyl (NP), a produced anti-NP antibodies having higher affinity for a cross-reactive analogue, (4-hydroxy-5-iodo-3-nitrophenyl) acetyl (NIP), than for NP itself (1). Furthermore, these "heteroclitic" antibodies share a set of common idiotypes, the predominant idiotype in primary B6 anti-NP antibodi...

antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. a promising alternative to conventional antibodies are the heavy chain antibodies (vhh) of camelidae having short length, high solubility and stability are preferred to other antibody derivatives. in this study, our goal was product...

We used a library of 31 monoclonal and six polyclonal antibodies to compare the structures of the two classes of cytoplasmic myosin isozymes isolated from Acanthamoeba: myosin-I, a 150,000-mol-wt, globular molecule; and myosin-II, a 400,000-mol-wt molecule with two heads and a 90-nm tail. This analysis confirms that myosin-I and -II are unique gene products and provides the first evidence that ...

Accumulated sequence analyses of the antibody repertoire have revealed that most autoantibodies and developmentally regulated antibodies share a small set of germline Ig-variable region (V) genes. The findings have prompted speculation that certain autoantibodies are of developmental importance and may be instrumental in maintaining homeostasis of the adult antibody repertoire. In order to eval...