The aerial parts of Artemisia vulgaris were subjected for successive extraction in solvents methanol, hexane and chloroform, respectively, compounds present extract analysed by GC-MS analysis 12 different found chloroform which Germacrene was most abundantly (33.84%). Chloroform showed strong antibacterial activity, examined against 6 bacteria using the disc diffusion method. IC50 value accounted...

This study aimed to examine the effects of repeated training on the controlled force exertion (CFE) test and retention in dominant and nondominant hands. The subjects were 10 young males (mean age, 21.8 ± 1.4 years). The CFE test was used as the repeated training task and performed 5 days per week over 3 weeks (a total of 15 times). The exertion value of the subjects’ handgrip strengths was mat...

Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole’s extraction mixture were obtained. Phospholipids are not extracted by the procedure descr...

Extraction and purification of Anthocyanin pigments from Black Raspberries (Rubus occidentalis) was successfully carried out. Homogeneous and fine powder was obtained from the liquid nitrogen blending process. After extraction and purification of the plant material, a colored solution was obtained. Depending on the nature of the predominant anthocyanin and its concentration, the coloration of t...

OBJECTIVES Cerebral fat embolism (CFE) is a serious complication after fracture of long bones. The mortality rate of CFE may be high. However, recent progress in treatment may decrease the mortality. We studied the validity of magnetic resonance imaging (MRI) to detect and grade severity of CFE in 11 patients with CFE. METHODS Glasgow Coma Scale score, PaO2, PaCO2 at the onset, and minimal he...

Both paraffin-embedded tissue specimens and buccal cells are excellent resources for large-scale molecular epidemiological studies. In order to identify the optimal method for DNA extraction, we compared three methods: (1) modified phenol-chloroform protocol; (2) simple boiling method; and (3) DNA Extraction Mini Kit. For paraffin-embedded tissue specimens, amplification of the beta-globin gene...

A human leukaemia cell line--HL-60--can be differentiated into neutrophils or macrophages and both differentiation processes are accompanied by changes of the lipid composition. Various methods were described for the extraction of lipids from cellular systems, but only two of them were applied to the HL-60 cell line so far. In this study we compared five selected extraction methods for the lipi...

AIM To evaluate the efficiency of phenol/chloroform, microwave, and Qiagen spin column based DNA extractions from paraffin wax embedded tissue for use in the polymerase chain reaction (PCR). In addition, to assess the reliability of amplifying a housekeeping gene to indicate successful viral DNA extraction. METHODS DNA samples extracted from 20 blocks of cervical carcinoma tissues using the t...

Changes in lipid levels/profiles can reflect health status and diseases. Urinary lipidomics, thus, has a great potential in clinical diagnostics/prognostics. Previously, only chloroform and methanol were used for extracting lipids from the urine. The present study aimed to optimize lipid extraction and examine differential lipid classes obtained by various extraction protocols. Urine samples we...