Cholinesterase and succinyldehydrogenase activity of surgically removed left atrial auricles from patients in atrial fibrillation and in sinus rhythm have been compared, using histochemical methods. Higher cholinesterase and lower succinyldehydrogenase activity has been found in atrial fibrillation than in sinus rhythm. The pulmonary capillary mean pressure of patients with atrial fibrillation ...

Atypical and usual human serum cholinesterases were purified and studied with the fluorescent probe, N-methyl-(7-dimethylcarbamoxy)quinolinium iodide. Four active sites per tetramer were found in each enzyme. The turnover numbers of usual and atypical cholinesterases were the same: 15,000 mumol of benzoylcholine hydrolyzed/min/mumol of active site; 48,000 min-1 for o-nitrophenylbutyrate; and 0....

The study of glioblastoma multiforme in the anterior chamber of the guinea pig eye provided an unusual opportunity to observe the effect of a malignant but locally confined neoplasm on a serum enzyme system. Biochemically, these tumor-bearing guinea pigs were distinguishable from normal controls by a significant depression of serum cholinesterase activity. This observation substantiated the pre...

Atypical and usual human serum cholinesterases were purified and studied with the fluorescent probe, N-methyl(7-dimethylcarbamoxy)quinolinium iodide. Four active sites per tetramer were found in each enzyme. The turnover numbers of usual and atypical cholinesterases were the same: 15,000 pmol of benzoylcholine hydrol~zedlminl~mol of active site; 48,000 min-’ for o-nitrophenylbutyrate; and 0.002...

The procedure of Dietz et al. (Clin. Chem. 19: 1309-1313, 1973) for plasma cholinesterase (EC 3.1.1.7) gives a background absorbance of 1.4 A when extended to erythrocyte cholinesterase (EC 3.1.1.8) measurement, because the peak absorbance of the reaction product, 5-thionitrobenzoate, coincides with the hemoglobin Soret band at 410 nm. Consequently, the precision of erythrocyte cholinesterase m...

BACKGROUND Several thiocholine alkanoyl esters were newly synthesized and explored as substrates for the assay of human serum cholinesterase after being subjected to the Ellman reaction (Arch Biochem Biophys 1958;74:443-50 and Arch Biochem Biophys 1959;82:70-7). METHODS We synthesized thiocholine ester iodides by the method of Renshow et al. (J Am Chem Soc 1938;60:1765-70). We examined solubi...

In a previous report (McNamara et at., 1954) it was noted that spinal reflexes could be abolished reversibly by anticholinesterase compounds. When the ammals were sacrificed after return of the reflex activity, some were found to have considerable cholinesterase in their spinal cords and in other areas of the body; in other animals the enzyme was apparently absent. No determinations of acetylch...

We report results of an investigation into the proficiency of cholinesterase (EC 3.1.1.8) phenotying, assessed in 13 laboratories between 1983 and 1986. Thirty-two specimens of serum were distributed for analysis: two, each in duplicate, from the eight genotypes that can be recognized by differential enzyme inhibitor numbers. The accuracy of genotype ascription was markedly improved over that o...