A previously developed method for screening organic acidurias by capillary electrophoresis has been validated for oxalate and citrate measurement in urine. Sample pretreatment is minimum, just acidification and centrifugation. Detection is by direct UV. Validation parameters of the method can be considered adequate. Response is linear for both analytes in standards and samples. The assayed rang...

Aim: The aim of the present study was to synthesize different sizes of gold nanoparticles (GNPs) and their characterization for use as a label in lateral flow assay particular for the detection of bluetongue in small ruminants. Materials and Methods: Size controlled synthesis of GNPs was done by using different concentration of sodium citrate. In this study, five different types of GNP were syn...

OBJECTIVE To compare the effectiveness of clomifene citrate plus metformin and clomifene citrate plus placebo in women with newly diagnosed polycystic ovary syndrome. DESIGN Randomised clinical trial. SETTING Multicentre trial in 20 Dutch hospitals. PARTICIPANTS 228 women with polycystic ovary syndrome. INTERVENTIONS Clomifene citrate plus metformin or clomifene citrate plus placebo. ...

In this work, a series of experiments investigate the evaporation gold nanofluid sessile droplets on perfluorodecyltrichlorosilane (PFTS) silicon substrate heated to 77 °C. The processes different same initial volume, all for 1% Cv volume concentration prepared from 0.1 mM original suspension, are visualized examine size effect nanoparticles (2.2, 5 and 10 nm) impact surface coating (with witho...

1. A method of preparation and purification of citrate oxaloacetate-lyase (EC 4.1.3.6) from Aerobacter aerogenes is described. 2. The equilibrium of this reaction has been determined at pH 8.4 and 25 degrees . It has been shown that K, i.e. [citrate(3-)]/[oxaloacetate(keto) (2-)][acetate (-)], is 3.08+/-0.72, but that K(app.), i.e. [total citrate]/[total oxaloacetate][total acetate], is markedl...

Lead, copper, cobalt, nickel, iron and zinc ions form monocitrate complexes (negative charge) in citrate solution which is absorbed on the citrate form of anion-exchange resins (citrate as counter ion) y exchange of citrate ion with citrate complexes. These are subsequently recovered from the resin by 0.5M ammonium citrate eluent at pH 2.0. Under optimized conditions, quantitative recovery ...

The simple, reliable and reproducible HPLC methods were developed for the analysis of Tadalafil and Sildednafil citrate(API). The column used was YMC-Pack ODS AQ (150 mm x 4.6 mm,i.d.).The mobile phase used was phosphate buffer (10mM,pH 3.0) acetonitrile gradient run at the flow rate of 1mL/min with UV (PDA) detector at 220nm at ambient temperature. Extraction of Tadalafil and Sildenafil citrat...

Aceclofenac is a non-steroidal anti-inflammatory drug (NSAID) that exhibits analgesic, antipyretic and anti-inflammatory activities. It is practically insoluble in water. The effect of hydrotropes such as urea and sodium citrate and blends (urea + sodium citrate) on the solubility of aceclofenac was investigated. The enhancement in the solubility of aceclofenac was more than 5 and 25 folds in 3...