Five different laboratory tests for the identification of Staphylococcus aureus were compared. Analyses of 271 presumptive S. aureus strains, supplemented with 59 well-defined methicillin-resistant S. aureus (MRSA) isolates, were performed. Only the Staphaurex Plus (Murex Diagnostics, Dartford, United Kingdom) and the Pastorex Staphplus (Sanofi, Marnes-La-Coquette, France) tests displayed 100% ...

For the elucidation of many of the problems of the nature and mode of action of staphylocoagulase, preparations of high biological activity and purity are essential. Preliminary studies along these lines have been reported in the literature.3 11,15 Thus, it has been shown that coagulase may be precipitated by alcohol, by acids, and by halfsaturation with ammonium sulfate. Walston, using 10 volu...

The study was carried out to investigate the prevalence and antimicrobial susceptibility of Coagulase-Negative Staphylococci isolated from Bovine Mastitis in and around Dharwad region. A total of 310 samples were screened and 180 confirmed Coagulase-Negative Staphylococci were obtained. The antimicrobial susceptibility of CoagulaseNegative Staphylococci against 10 antimicrobial agents was teste...

Borchardt, Kenneth A. (Tulane University School of Medicine, New Orleans, La.), and William A. Pierce, Jr. In vitro phagocytosis of Staphylococcus aureus with special reference to a possible antiphagocytic function of coagulase. J. Bacteriol. 87:311-315. 1964.-Rabbit antisera were prepared to Staphylococcus aureus strains of bacteriophage types 81 and 42D with vaccines consisting of washed, hea...

Lysozyme production is a frequent property of potentially pathogenic staphylococci. In the present study, 1,186 strains of human origin, 85 strains of animal origin, and 156 strains of Staphylococcus albus (epidermidis) were tested. Of 1,114 coagulase-positive strains of human and animal origin, 1,098 were lysozyme-positive (98.5%). On the other hand, of 157 coagulase-negative strains which, ba...

OBJECTIVES Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance. METHODS Hundred clinical isolates of coagulase-negative staphylococci were characterized for integ...

Factors obtained from certain S variants of Staphylococcus aureus can both increase the potency of and destroy the coagulase produced by R variants of the organism. Evidence that separate factors are responsible for potentiation and for destruction of coagulase is based on differences of pH optimum and of temperature range of the factors. Potentiation of coagulase is most marked below pH 7 and ...

OBJECTIVES The emergence of antibiotic-resistant bacteria such as Staphylococcus aureus calls for inventive research and development strategies. Inhibition of bacterial pathogenesis may be a promising therapeutic approach in this regard. The gene-silencing effect of short interfering RNA (siRNA) is useful for this strategy. We investigated the efficacy of siRNA on the expression of coagulase be...

Correlation between the pathogenicity of certain staphylococci and their ability to produce coagulase has led to extensive studies of the clotting enzyme. Walston (1935) precipitated the clotting substance from cell-free filtrates of appropriate staphylococcal cultures by the use of ethanol, acetic acid, or ammonium sulfate. Walker et al. (1948) partially purified staphylocoagulase by repeated ...

A new scheme for identification of coagulase-negative staphylococci was applied to 138 consecutive urinary isolates of coagulase-negative staphylococci. The most common species were Staphylococcus epidermidis (53%), S. hominis (12%), and S. haemolyticus (10%). S. saprophyticus comprised only 5%. The disk method for antibiotic susceptibility for all species grouped together disclosed resistance ...