G-Protein Subunit of Cochliobolus heterostrophus Involved in Virulence, Asexual and Sexual Reproductive Ability, and Morphogenesis Sherif Ganem,†‡ Shun-Wen Lu,† Bee-Na Lee,§ David Yu-Te Chou,¶ Ruthi Hadar, B. Gillian Turgeon, and Benjamin A. Horwitz* Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel, Department of Plant Pathology, Cornell University, Ithaca, New York...

The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple mediu...

The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was ob...

A reação de biotransformação da digitoxigenina (1) por Cochliobolus lunatus foi investigada. Foram realizados experimentos com duração de 4 dias, que resultaram no isolamento de quatro produtos, cujas estruturas químicas foram elucidadas como sendo 1β-hidroxidigitoxigenina (2), 7β-hidroxidigitoxigenina (3), 8β-hidroxidigitoxigenina (4) e digitoxigenona (5). A obtenção desses produtos nas condiç...

Maize Hm1 gene encodes a NAD(P)H-dependent HC-toxin reductase, which detoxify HC-toxin produced by fungus Cochliobolus carbonum (Meeley and Walton 1991 Plant Phys 97: 1080). Measurements of ion leakage indicated that H2O2 treatment of a recessive mutant (hm1) of maize resulted in accelerated death in excised leaves. Furthermore, an hm1 maize showed quantitative decrease of NAD(H) level. Thus, t...

Cochliobolus heterostrophus race T, causal agent of southern corn leaf blight, requires T-toxin (a family of C35 to C49 polyketides) for high virulence on T-cytoplasm maize. Production of T-toxin is controlled by two unlinked loci, Tox1A and Tox1B, carried on 1.2 Mb of DNA not found in race O, a mildly virulent form of the fungus that does not produce T-toxin, or in any other Cochliobolus spp. ...

Trichoderma asperellum is one of the plant growth-promoting fungi (PGPF) presently marketed as biopesticide, biofertilizer and in soil amendments, due to its ability protect plants, enhance vegetative growth reduce pathogen populations. Keeping this view, here, we assessed efficacy against pathogens medicinal plants vitro field conditions Leaf spot disease Green leaf Tulsi (Ocimum basilicum). W...