Water soluble polymers such as Hydroxy Propyl Methyl Cellulose (HPMC), Poly Vinyl Pyrrolidone (PVP), Carboxy Methyl Cellulose Sodium (CMC Na), and Methyl Cellulose as well as in combination of varying proportions are used in this study for the protective coating of Microcrystalline Hydroxy Apatite Complex (MCHC). The objective of this study was to investigate the effect of aqueous film coating ...

the aim of this work was to investigate the effect of silica coating on bioactivity and biodegradability of hydroxyapatite. in this purpose, we firstly attempted to synthesis hydroxyapatite (ha) nanoparticles and its silica coated (si-ha) sample in collagen matrix using calcium chloride, sodium phosphate and sodium silicate. characterization of the sample was carried out using fourier transform...

Reconstruction of tendon and ligament tissues requires proper attachment of the tissue-engineered construct to surrounding tissues. A problem of reconstructing collagen-rich tissues is that an in vitro engineered collagenous network containing fibroblasts will contract and detach from a solid surface. In vivo anchorage of soft connective tissues to mineralized tissues like bones and teeth is ac...

INTRODUCTION The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. PURPOSE To i...

Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods use glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for binding collagen type I covalently on P...

Primary cancer cells constitute a favourable testing platform for in vitro research in oncology field as they reflect tumour state more accurately than the most commonly employed stable cell lines. Unfortunately, due to limited availability of material and difficulties with protocols validation, primary models are rarely implemented into laboratory practice.We have compared protocols for primar...

PURPOSE Primary mammalian hepatocytes largely retain their liver-specific functions when they are freshly derived from donors. However, long-term cultures of functional hepatocytes are difficult to establish. To increase the longevity and maintain the differentiated functions of hepatocytes in primary culture, cells can be cultured in a sandwich configuration of collagen. In such a configuratio...

For particular cell-based therapies, it may be required to culture mesenchymal stem cell (MSC) aggregates with growth factors to promote cell proliferation and/or differentiation. Heparin, a negatively charged glycosaminoglycan (GAG) is known to play an important role in sequestration of positively charged growth factors and, when incorporated within cellular aggregates, could be used to promot...