Erythroid progenitor cells, CFU-E (colony-forming-unit-erythroid), were isolated to practical homogeneity by a combination of three enrichment procedures. CFU-E were generated in large amounts in spleens of mice previously bled and treated with the erythropoiesis-suppressing drug thiamphenicol. The average CFU-E concentration in spleens from mice 4 d after the thiamphenicol-treatment was 10%. T...

The best practice for cleansing urinary drainage bags (leg and bed) in a hospital setting can be translated for use by clients for self-care within the home setting. Common household bleach containing 5.25% sodium hypochlorite, diluted in a 1:10 ratio with tap water, is a safe and effective method to reduce colony-forming units per milliliter (CFU/ml) to a study-defined acceptable range of 100 ...

Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method ...

Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method ...

Antibody L4F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM, CFU-Meg, BFU-E, and CFU-Mix. In the present study we examined whether cells that do not express the L4F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with L4F3 and com...

Indigenous microflora community changes during six months of co-composting of activated sewage sludge and date palm waste was investigated using two different culture approaches. In order to evaluate the co-composting process evolution for mixture A and B, growth standard media (GSM) and Compost Time Extract Agar (CTEA) are used. Enumeration for indigenous flora abundance on GSM medium shows th...

OBJECTIVE To compare the number of bacterial counts in tap water at first burst and running tap water. MATERIAL AND METHOD The present study was performed in thirty-two first burst water samples and twenty-nine running tap water samples after two minutes, collected from an operation room at one standard hospital and analyzed for the mean, median, and 95% achieved confidence interval of CFU/ml...

In this study, the culturability of indoor and outdoor airborne fungi was determined through long-term sampling (24-h) using a Button Personal Inhalable Aerosol Sampler. The air samples were collected during three seasons in six Cincinnati area homes that were free from moisture damage or visible mold. Cultivation and total microscopic enumeration methods were employed for the sample analysis. ...

BACKGROUND The Dijon University Hospital in Dijon, France is involved in a large construction program with heavy truck traffic and a very dusty environment. This study aimed to assess the impact of outdoor hospital construction work on Aspergillus air contamination in the immediate environment of patients at high risk for aspergillosis in the presence of protective measures. METHODS Prospecti...

We investigated Staphylococcus aureus contamination of the hand rims and handles of wheelchairs for common use on hospital wards by wiping with gauze. Examination of the hand rims of a total of 20 wheelchairs revealed methicillin-sensitive S. aureus (MSSA) and/or methicillin-resistant S. aureus (MRSA) on 17 wheelchairs (85.0%), and MRSA on 11 (55.0%). The mean ± SD (range) density of MRSA conta...