Antiserum was prepared in rabbits against the A antigen and a new antigen (labeled the D antigen) of yeast-phase cells of Blastomyces dermatitidis by using immunoelectrophoretic precipitin arcs as vaccine. The antiserum can be used as a reference serum in the immunodiffusion test for identyfying antibodies to the A antigen in sera from patients thought to have blastomycosis. Antibodies to the D...

An immunodiffusion technique in agarose is described for assay of complementfixing antibodies against the type-specific soluble antigen of influenza virus. Under the test conditions, positive human serum produced a definite unlysed zone around the well, and the annulus area showed a high correlation with the antibody level in a conventional complement fixation test with log2 serum titer. This p...

i. Radiological examination of chest, teeth, sinuses, and lumbo-sacral joints. 2. Blood tests including a full blood count, erythrocyte sedimentation rate (ESR), antistreptolysin titre, mucoprotein levels, blood sugar levels, gonococcal complement-fixation test, toxoplasma dye and complement-fixation tests, Rose latex test for arthritis, serum protein electrophoresis, histoplasma complement-fix...

Immune adherence hemagglutination was compared with the complement fixation test as a means of measuring antibodies to varicella-zoster virus. Analysis of acute- and convalescent-phase sera from patients infected with varicella-zoster or with herpes simplex virus showed the immune adherence hemagglutination test to be more sensitive than the complement fixation test, and greater cross-reactivit...

A micro complement fixation assay was devised to measure the type-specificity of anti-M antibody. Unabsorbed sera were from rabbits hyperimmunized with heat-killed streptococci and from humans with naturally occurring antibody or immunized with purified M protein vaccines. These sera fixed complement only in the presence of homologous M proteins (serotypes 1, 3, 6, 12, and 14). The complement f...

Investigation was conducted on (90) serum samples, (47) samples were collected from aborted cows and (43) samples from non-aborted cows from Babylon governorate, there were 7, 14.89% cases infected with Brucellaabortus by iELISA test while 10, 21.27% and 9, 19.14% cases infected with Brucellaabortus by Complement fixation test and rose Bengal plate test respectively from infected cow, there wer...

In a previous paper, Tringali, Del Carpio, and Gismmanco (1966) reported that treatment of sera with 2-Mercaptoethanol (2-ME) caused a disappearance of non-syphilitic anticardiolipin reactivity, usually referred to as T'false positive" (EP) reactivity, as detected in a complement-fixation assay, However, similar treatment did not abolish the reactivity of syphilitic sera in the samie assay. Thi...

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Antigen from purified Mycoplasma pneumoniae organisms treated with Tween-80-ether was used in a solid phase enzyme immunoassay and compared with the conventional lipid containing complement fixation antigen for measuring antibodies in sera from patients with aseptic or bacterial meningitis or with apparent M pneumoniae infection. In immunoblotting of the enzyme immunoassay antigen, enzyme immun...