The study was carried out on healthy Friesian calves (n = 10) aged between 10 and 30 days. Hypochloremia and alkalosis were induced by intravenous administration of furosemide and isotonic sodium bicarbonate. The venous and arterial blood samples were collected repeatedly. 2,3-diphosphoglycerate (2,3-DPG), hemoglobin and plasmatic chloride concentrations were determined. The red blood cell chlo...

A new hypothetical mechanism is proposed for photoassembly of the (Mn)4-cluster of the photosynthetic oxygen evolving complex (OEC). In this process, a neutral radical of Y(Z) tyrosine plays a role in oxidizing Mn2+ associated with an apo-OEC, and also in abstracting a proton from a water molecule bound to the Mn2+ ion, together with D1-His190. This is in a similar fashion to the metallo-radica...

Within photosynthetic organisms, the oxygen-evolving complex (OEC) of photosystem II generates dioxygen from water using a catalytic Mn(4)CaO(n) cluster (n varies with the mechanism and nature of the intermediate). We report here the rational synthesis of a [Mn(3)CaO(4)](6+) cubane that structurally models the trimanganese-calcium-cubane subsite of the OEC. Structural and electrochemical compar...

Efficient photoelectrochemical water oxidation may open a way to produce energy from renewable solar power. In biology, generation of fuel due to water oxidation happens efficiently on an immense scale during the light reactions of photosynthesis. To oxidize water, photosynthetic organisms have evolved a highly conserved protein complex, Photosystem II. Within that complex, water oxidation happ...

The oxygen evolving complex (OEC) of photosystem II (PSII) requires chloride as a critical cofactor. The Cl content of PSII was determined to be 1 Cl/PSII by the AgCl colloid assay. The dissociation constant for Cl in NaCl-washed PSII was determined using the Cl sensitive microelectrode. Two classes of Cl binding sites were found with Kd values of 53 μM and 4.2 mM. The effects of anions on the ...

INSEMEX-OEC is a Conformity Assessment Body for certification activities in accordance with ATEX Directive 2014/34/EU, being accredited by RENAR and notified to Brussels (NB1809). INSEMEXGLI (Group of Testing Laboratories) the main provider laboratory testing services product body INSEMEX-OEC. The test laboratories meet requirements standard SR EN ISO / IEC 17025. This requires have procedure p...

It is an important task to automatically and accurately map rooftops from very high resolution remote sensing images since buildings are closely related human activity. Two typical technologies often utilized accomplish the task, i.e., semantic segmentation instance segmentation. The independently allocate a label (e.g., “building” or not) each pixel, resulting in blob-like segments. On contrar...

Bergman complexes are polyhedral complexes associated to matroids. Faces of these complexes are certain matroids, called matroid types, too. In order to understand the structure of these faces we decompose matroid types into direct summands. Ardila/Klivans proved that the Bergman Complex of a matroid can be subdivided into the order complex of the proper part of its lattice of flats. Beyond tha...

F rom ancient times through the 19th century, physicians used bloodletting to treat acne, ca ncer, diabetes, jaundice, plague, and hundreds of other diseases and ailments (D. Wooton, Doctors Doing Harm since Hippocrates, Oxford Univ. Press, 2006). It was judged most effective to bleed patients while they were sitting upright or standing erect, and blood was often removed until the patient faint...