Laser induced isotopic fractionation Previous studies of laser ablation (LA) MC-ICP-MS analysis of Cu metal and sulphides showed reduced Cu/Cu ratios compared to solution nebulisation MC-ICP-MS of the same samples [1]. The fractionation of about 30ε (parts per 10,000) was reproduced repeatedly and observed, to a smaller degree, for Ag and Sb also. This deviation, which was attributed largely to...

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Heavy metals are potentially toxic to human life and the environment. Metal toxicity depends on chemical associations in soils. For this reason, determining the chemical form of a metal in soils is important to evaluate its mobility and hioavailability . Sequential extraction was used to fractionate four heavy metals (Cd, Cu, Ni, and Zn) from nine contaminated soils into six operationally defin...

This study provides a zinc and copper stable isotope characterization (δ66Zn, δ68Zn, δ65Cu) of metalliferous seafloor sediments from the Atlantis II Deep, hydrothermally influenced brine basin in Red Sea. Samples collected box cores that capture entire stratigraphy Deep have δ66Zn δ65Cu values −0.31 to 0.34 ‰ (0.02 median) − 1.81 1.02 (−0.34 relative JMC-Lyon NIST SRM 976 standards, respectivel...

When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues, is injected intravenously into a rat, more than half of the radioactivity can be recovered within minutes in ASCPN precipitated immunochemically from liver homogenates. Both radioautographic studies and measurements of hepatic uptake of ASCPN following blockade of the reticuloendothelial system ind...

The p36 and p95 proteins of Carnation Italian ringspot virus (CIRV), when expressed in Saccharomyces cerevisiae, supported the replication of defective interfering (DI) RNA. Double-label confocal immunofluorescence showed that both proteins localized to mitochondria, independently of each other. DI RNA progeny was localized by in situ hybridization both to mitochondria and to their proximity. F...

The impact of anaerobic digestion on the bioavailability of copper and zinc from pig slurry was assessed. Both chemical and biological approaches were used independently on raw slurry (RS) and anaerobically digested pig slurry (DS). This work, using ultracentrifugation pellets from the same pig slurry before and after an anaerobic treatment, confirmed that Cu and Zn behave differently in terms ...

A convenient method for direct photometric determination of total globulin in serum has been developed using a one-tube, one.reagent system. The analysis is based on a variation of the Hopkins-Cole reaction, in which glyoxylic acid condenses with the tryptophan residues present in globulin to produce a purple color. In the presence of copper and under the conditions of the analysis, color inten...

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...

BACKGROUND The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reporte...