Spontaneous cross-linking in vitro, during incubation of purified collagen in nonstriated and native type fibrils, was studied to elucidate the relationship between intra and intermolecular cross-links. In nonbanded fibrils, the aldol condensation products of two allysyl residues, previously shown to constitute the intramolecular cross-link, formed spontaneously and could be isolated as such, w...

At NTCIR-9, we participated in the cross-lingual link discovery (Crosslink) task. In this paper we describe our approaches to discovering Chinese, Japanese, and Korean (CJK) cross-lingual links for English documents in Wikipedia. Our experimental results show that a link mining approach that mines the existing link structure for anchor probabilities and relies on the “translation” using cross-l...

Chemical cross-links identified by mass spectrometry generate distance restraints that reveal low-resolution structural information on proteins and protein complexes. The technology to reliably generate such data has become mature and robust enough to shift the focus to the question of how these distance restraints can be best integrated into molecular modeling calculations. Here, we introduce ...

This paper describes the evaluation in benchmarking the effectiveness of cross-lingual link discovery (CLLD). Cross-lingual link discovery is a way of automatically finding prospective links between documents in different languages, which is particularly helpful for knowledge discovery of different language domains. A CLLD evaluation framework is proposed for system performance benchmarking. Th...

The effect of the single, site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of a 20-base pair DNA duplex is reported. The cross-linked or unplatinated 20-base pair duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption, and circular dichroism. The cross-link of both platinum ...

Viscoelastic relaxation processes factor into polymer performance and stability throughout an application lifetime, controlled by the polymer network structure and dynamics which occur over a wide spectrum of time scales. In this work, we detail the design and operation of an independent array of surface indenters which can measure the creep response at multiple points on a polymer substrate. S...

An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions. The key variables studied are the cell suspension hold time prior to centrifugation, the relative centrifugal force (RCF), time of centrifugation, cell pellet resuspension velocities, and number of resuspension passes. The...

Bomb calorimetry measurements were made on dried whole body samples of N. guentheri in an attempt to detect the effect of age induced protein crosslinking. The results show a clear decrease in body energy with increasing age. However, this decrease was larger than expected and was probably due to a slight decrease in body fat component with increasing age rather than crosslinking alone.