background herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are common infectious agents worldwide. data on prevalence of hsv-1 and hsv-2 infections are limited in asia, especially in iran. our study aimed on determination of seroepidemiology of hsv-1 and hsv-2 infections in asymptomatic healthy students of islamic azad university of kazeroun, fars province, southwest of iran. patients ...

results: of the 50 samples tested by the elisa method, 26 samples (52%) were positive for igg . no samples were positive for igm. of the 50 samples tested by the chemiluminescence method, 28 samples (56%) were positive for igg. no samples were positive for igm. conclusions: a significant relationship between the age of the youngest child and the infection rate was seen. no significant correlati...

an enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. immunoglobulin fractions were purified and coated on wells of microtiter plates. a chain heterology was introduced in neopterin derivative and conjugated to penicillinase. the assay is...

Lipases are the most widely employed enzymes in commercial industries. The catalytic mechanism of most lipases involves a step called "interfacial activation". As interfacial activation can lead to a significant increase in catalytic activity, it is of profound importance in developing lipase immobilization methods. To obtain a potential biocatalyst for industrial biodiesel production, an effec...

Clostridium cylindrosporum formyltetrahydrofolate synthetase tetramers cross-linked with dimethyl suberimidate remained active in the absence of the monovalent cations normally required for enzymic activity and the tetrameric conformation. The modified enzyme was analyzed by sodium dodecyl sulfate electrophoresis, sedimentation velocity, and gel permeation chromatography. Under the experimental...

In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobili...

A cross-linked mutant endoglucanase II was prepared for enzymatic polymerization to cellulose. The cross-linked enzyme is composed of three mutant enzymes showing polymerization activity. A characteristic feature of the polymerization with this cross-linked enzyme is formation of cellulose fibrils in contrast to plate-like crystals obtained by using a free enzyme.

?-Glutamyl transpeptidase (GGT) catalyzes the transfer of glutathione’s ?-glutamyl group and related amides to water, amino acids or peptides, utilizes a conserved Thr residue process its own polypeptide chain into large small subunit that then assemble produce catalytically competent enzyme. In this study, magnetic cross-linked enzyme aggregates (mCLEAs) transpeptidase-specialized variant (N45...