This study describes molecular basis for positive B-cell flow crossmatch in a zero mismatch (0MM) transplant pair. Our end-stage renal disease patient was B-cell flow crossmatch positive with a 0MM deceased donor. DNA from the donor and patient were further typed by a high-resolution method. The patient’s sera were tested for anti-HLA reactivity by single antigen bead using a Luminex platform. ...

Serum samples from 49 patients with panel reactive antibodies of greater than 15% and 17 patients who have related donor pairs were collected at the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Crossmatching was performed by three methods, flow cytometry crossmatch (FCXM), the standard National Institutes of Health (NIH), and the antihuman globulin (AHG) microlympho...

A crossmatch test between patient and donor’s blood must be carried out before transfusion. However, not all crossmatched will transfused to the patient. This indicates inappropriate distribution of products, wastage, inaccessibility for emergency patients, increased costs, workload staff. Crossmatch Transfusion Ratio (C/T Ratio) is used as an indicator determine efficiency use can serve a guid...

A 56-year-old white female presented for evaluation for kidney transplantation in November 2003. Her end-stage renal disease (ESRD) was presumed due to medullary sponge kidney associated with stones and infection and she had started haemodialysis in April 2003. Other medical history included hypertension and anaemia. She was otherwise well. She had had four pregnancies and had received blood tr...

in this paper, for the first time, blood inventory-routing problem (irp) is defined and a mixed integer linear programming formulation is presented. to do so, the concepts of assigned inventory, transfusion to crossmatch ratio, crossmatch release period, older-first policy and order-up-to level inventory policy along with perishability considerations are incorporated into the latest inventory-r...

Even in this era of efficient immunosuppression, a positive serological crossmatch caused by complement fixing antibodies directed against the human leukocyte antigen (HLA) mismatches of the organ donor is a contraindication for transplantation (1). The presence of this type of antibodies is known to be associated with a high incidence of hyper acute or acute-accelerated rejection (2). Highly s...

Although hyperacute rejection has been clinically and pathologically fully described in recipients of other solid organ transplants, to our knowledge, there have been no previous fully documented cases in recipients of lung transplants. This case of clinical hyperacute rejection is corroborated by a positive, donor-antigen-specific IgG-mediated lymphocytotoxic crossmatch (LXM), and demonstrated...

Substances occurring naturally in the sera of patients can interfere with Luminex antibody assays, causing increased background and changes in antibody specificity. We present data on the effectiveness of hypotonic dialysis (HD) or dithiothreitol (DTT) treatment in eliminating this interference. HD significantly increased reaction strength of positive control beads and reduced reaction strength...