A monoclonal IgM antibody (18-2-3) derived from cell fusion of (NZB X NZW) F1 splenocytes following secondary immunization with fluorescein-conjugated keyhole limpet hemocyanin was shown to exhibit high intrinsic binding affinity and cryoinsolubility. Affinity-purified preparations were determined to be IgM by immunochemical, electrophoretic, and chromatographic analyses. An intrinsic associati...

Case report A 66-year-old female presented with a 3 years history of multiple ulcerations on the lower extremities of both legs. The diagnosis of necrotic angiodermitis had been previously proposed. The skin lesions were numerous (6 on right side and 7 on left side), purpuric, evolutive and painful, with width ranging from 0.5 to 4.5 centimeters. No adenopathy, neither liver or spleen enlargeme...

A rapid screening method for serum cryoblobulin is reported. It requires only common laboratory equipment and is based upon the detection of light scattering (500 millimicron) in the early phase of cryoaggregation. All of 28 sera negative for cryoglobulins by the conventional 5-day incubation method were negative by the screening method. Conversely, all sera containing 60 microgram/ml or more o...

Key words: IgG cryoglobulinaemia; leukocytoclastic festations in their study of 21 patients with type I cryoglobulinaemia in 25% of cases. The renal mani-vasculitis; monoclonal cryoglobulinaemia; membrano-proliferative glomerulonephritis (MPGN) festations may result from massive subendothelial deposits of cryoglobulins, or diffuse glomerulonephritis with subendothelial deposits similar to those...

Cryoglobulinaemia refers to circulating cryoglobulins. Cryoglobulins are immunoglobulins, which precipitate in temperatures below 37oC and dissolve upon rewarming [2]. Cryoglobu‐ linaemia is classified in 3 types based on clonality and immunoglobulin class. In particular, type I consists of monoclonal IgM or IgG immunoglobulin, type II is a mixture of monoclo‐ nal IgM and polyclonal IgG, while ...

Four cases showing different means to detect cryoglobulins are reported: effects on blood cell counts performed on 2 technologically different automated hematology instruments and microscopic features in fresh blood samples and on May-Grünwald-Giemsa-stained bloodfilms. These cases were chosen for their instructive value in depicting all artifacts associated with cryoglobulins. Laboratory recog...