Cryopreservation of avian ova and embryos is challenging because of the yolky structure of the egg. As an alternative, with the development of effective cryopreservation protocols, ovarian tissue cryopreservation could be used for cryobanking for birds. Pieces of ovarian tissue of week-old Japanese quail (Coturnix japonica) were frozen at 0.5 degrees C/min in a programmable freezer or vitrified...

BACKGROUND Ovarian tissue cryopreservation with later reimplantation has been shown to preserve fertility in adult women, but this approach remains unproven and experimental in children and adolescents. We aimed to assess the use of the Edinburgh selection criteria for ovarian tissue cryopreservation in girls and young women with cancer to determine whether we are offering this invasive procedu...

BACKGROUND Ovarian tissue cryopreservation may be a potential method of preserving fertility in women who have experienced gonadotoxic treatments. To improve the efficiency of existing cryopreservation, we developed a practical and convenient vitrification method named needle immersed vitrification (NIV), which required a less concentrated and minimum volume of vitrification solution. METHODS...

Cancer treatments can induce premature ovarian failure in almost half of young women suffering from invasive neoplasia. Cryopreservation of ovarian cortex and subsequent autotransplantation of frozen-thawed tissue have emerged as promising alternatives to conventional fertility preservation technologies. However, human ovarian tissue is generally harvested before the administration of gonadotox...

At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under constant agitation and culture on embryonic chorio-allantoic membrane within a hen's eggs. The ai...

In this issue of Blood, Greve et al report that ovaries from leukemia patients in complete remission do not appear to contain viable malignant cells, in contrast to ovarian tissue retrieved before chemotherapy.1 This paper raises a number of questions because 3 different teams (Meirow et al,2 Rosendahl et al,3 and Dolmans et al4) have so far demonstrated by PCR that ovarian tissue from leukemia...