The terrestrial anuran Rana sylvatica tolerates extended periods of whole-body freezing during the winter. Freezing survival is facilitated by extensive glycogen hydrolysis and distribution of high concentrations of the cryoprotectant glucose into blood and all tissues. As glycogenesis is both an energy-expensive process and counter-productive to maintaining sustained high cryoprotectant levels...

Gene banking is arguably the best method available to prevent the loss of genetic diversity caused by declines in wild populations, when the causes of decline cannot be halted or reversed. For one of the most impacted vertebrate groups, the amphibians, gene banking technologies have advanced considerably, and gametes from the male line can be banked successfully for many species. However, cryop...

The field of cryobiology originated in 1949, when Polge, et al., described the cryoprotective property of glycerol in the freezing of spermatozoa (1). Lovelock and Bishop reported the cryoprotective ability of dimethyl sulfoxide (DMSO) on red blood cells in 1959 (2). Fortytwo years later, DMSO was used to preserve the first cryogenically-stored cells launched onboard STS-105 for use on the firs...

The study aimed to determine the effects of different concentrations of glycerol and ethylene glycol and ultrarapid cryopreservation (vitrification) on survival and subsequent in vitro development of bubaline cumulus oocyte complexes (COCs) in order to recognize the optimum cryoprotectant. Survival and in vitro maturation, fertilization and cleavage of buffalo COCs was evaluated subsequent to t...

ABSTRACT: Toxoplasma gondii can be eliminated in bovine semen. Cryopreserved semen is often used due to the fact that artificial insemination dairy and beef cattle provides benefits terms of production. However, little known regarding viability infectivity T. tachyzoites cryopreserved In present study, negative for were contaminated with 1 x 106 (RH strain) without different cryoprotectants, su...