Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 A. The crystals belonged to space group P6(1)22 or P6(5)22...

The asymmetric unit of the title compound, C(15)H(13)BrN(2)O(3)·0.5CH(3)OH, contains two Schiff base mol-ecules and a methanol mol-ecule of crystallization. The dihedral angles between the benzene rings in the two mol-ecules are 23.8 (2) and 18.6 (2)°. In the crystal structure, mol-ecules are linked through inter-molecular N-H⋯O, O-H⋯O and O-H⋯N hydrogen bonds, forming a three-dimensional network.

Batch crystallization is an important chemical unit operation. So, large numbers of published works were focused on batch modelization, simulation, optimization, control and parameter estimation. In this work, optimization made with the objective to obtain optimal cooling temperature strategy a crystallizer realized in our laboratory. The potassium hydrogenotartarate (cream tartar) subject stud...

Cisplatin and carboplatin are platinum anticancer agents that are used to treat a variety of cancers. Previous X-ray crystallographic studies of carboplatin binding to histidine in hen egg-white lysozyme (HEWL) showed a partial chemical conversion of carboplatin to cisplatin owing to the high sodium chloride concentration used in the crystallization conditions. Also, the co-crystallization of H...

Adeno-associated virus serotype 5 (AAV5) is under development for gene-therapy applications for the treatment of cystic fibrosis. To elucidate the structural features of AAV5 that control its enhanced transduction of the apical surface of airway epithelia compared with other AAV serotypes, X-ray crystallographic studies of the viral capsid have been initiated. The production, purification, crys...

in this study, the effect of recrystallization of naproxen in the presence of hydroxypropyl cellulose (hpc) on the release rate of drug was investigated. crystals were generated by the anti-solvent approach using the hpc solution in water as the anti-solvent. the samples were subjected to various physicochemical evaluations such as crystal size, scanning electron microscopy, fourier transform i...

Chorismate mutase catalyzes the conversion of chorismate to prephenate in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and plants. Here, the crystallization of the unusual secreted chorismate mutase from Mycobacterium tuberculosis (encoded by Rv1885c), a 37.2 kDa dimeric protein belonging to the AroQ(gamma) subclass of mutases, is reported. Crystal ...

Adeno-associated viruses (AAVs) are actively being developed for clinical gene-therapy applications and the efficiencies of the vectors could be significantly improved by a detailed understanding of their viral capsid structures and the structural determinants of their tissue-transduction interactions. AAV8 is approximately 80% identical to the more widely studied AAV2, but its liver-transducti...

Protein crystallization is frequently induced by the addition of various precipitants, which directly affect protein solubility. In addition to organic cosolvents and long-chain polymers, salts belong to the most widely used precipitants in protein crystallography. However, despite such widespread usage, their mode of action at the atomistic level is still largely unknown. Here, we perform exte...