2007 saw the publication of two molecular phylogenies of Cucumis that relied on combined chloroplast and nuclear gene regions, and broad species sampling. One of these studies also addressed the position of Cucumis in the Cucurbitaceae family tree. It was found that the genera Cucumella, Dicaelospermum, Mukia, Myrmecosicyos, and Oreosyce are embedded within Cucumis and that the closest relative...

To prepare tissues for analysis of NAD(+), NADH, NADP(+), and NADPH, common practice is to freeze samples in liquid nitrogen, often followed by freeze-drying, before extraction in HCl or NaOH. With cucumber (Cucumis sativus L.) cotyledons, prefreezing in liquid nitrogen or slower freezing to -20 degrees C yielded substantially lower values for NADH and NADPH than obtained from samples homogeniz...