of Alcaligenes eutrophus. the acetyl coenzyme A synthetase gene (acoE)

Renewable resources constitute a ‘limited infinity’, meaning that a limited amount is being produced, but for an infinite time.1 One of the most promising approaches to reduce the resource intensity is to use the existing biomass more efficiently: the biorefinery concept. It aims to use the complete biomass and all by-products emitted by the processes. Particularly the by-products of establishe...

Despite its advantages over synthetic polymers from petroleum-based feed-stocks, microbially produced poly-β-hydroxybutyrate (PHB) is not commercially competitive owing to low productivities. Productivity enhancement has been explored here through in silico experiments with Wautersia eutropha in fed-batch fermentations utilizing fructose as the carbon substrate and urea to provide nitrogen. The...

The thermophile Cupriavidus sp. strain S-6 accumulated polyhydroxybutyrate (PHB) from glucose at 50°C. A 9.0-kbp EcoRI fragment cloned from the genomic DNA of Cupriavidus sp. S-6 enabled Escherichia coli XL1-Blue to synthesize PHB at 45°C. Nucleotide sequence analysis showed a pha locus in the clone. The thermophilic polyhydroxyalkanoate (PHA) synthase (PhaC(Csp)) shared 81% identity with mesop...