AIM To develop an in vitro intact cell-based assay for screening selective cyclooxygenase inhibitors. METHODS Human cyclooxygenase-1 (hCOX-1) and cyclooxygenase-2 (hCOX-2) genes were cloned from human monocyte cell line THP-1 cells and expressed in Spodoptera frugiperda (sf9) insect cell line by Bac-to-Bac baculovirus expression systems. Infected sf9 cells were harvested 24 h post-infection (...

Cyclooxygenase and 5-lipoxygenase are enzymes that catalyze important inflammatory pathways, suggesting that dual cyclooxygenase/lipoxygenase inhibitors should be more efficacious as anti-inflammatory medicines with lower side effects than the currently available nonsteroidal anti-inflammatory drugs. Many plants from the family Asteraceae have anti-inflammatory activities, which could be exerte...

arthritis refers to different medical conditions associated with disorder of the primary structures that determine joint functions such as bones, cartilage and synovial membranes. drug discovery and delivery to retard the degeneration of joint tissues are challenging. current treatments of different arthritis involves administration of ideal non-steroidal anti-inflammatory drugs (nsaids) but ar...

Growth factors and tumor promoters have been shown to play a role in intestinal epithelial growth regulation and transformation. In this study, transforming growth factor-alpha (TGF alpha) and the tumor promoter, tetradecanoyl phorbol acetate (TPA), are shown to stimulate the production of eicosanoids by rat intestinal epithelial (RIE-1) cells in culture. A 4.5-kb mRNA, which hybridizes to the ...

Prostaglandin-endoperoxide synthase 2 is the rate-limiting enzyme in the biosynthesis of prostaglandins (PGs). In this paper, we briefly introduced the structure, expression, regulation mechanism of prostaglandinendoperoxide synthase 2 gene and the relationship between this gene and reproductive performance.

In this study, we focused on screening and simulating the interaction between anti-inflammatory proteins 50 compounds isolated from Millettia dielsiana Harms ex Diels. 39 out of that violated no Lipinski’s rule five were sorted as favorable for drug development selected studies further. Then, a molecular docking study into binding sites COX-1 COX-2 allowed shedding light mode these potential CO...

OBJECTIVE To review proper use of gastroprotective strategies in family medicine for patients requiring chronic nonsteroidal anti-inflammatory drug (NSAID) therapy. QUALITY OF EVIDENCE Evidence of the efficacy and safety of strategies currently in use (prostaglandin analogues, cyclooxygenase-2 inhibitors, proton pump inhibitors) is derived from randomized controlled trials (level I evidence)....