T HE CYTOPLASMIC GRANULES of eosinophils may be considered a form of lysosome, for they are membrane bounded structures containing, among other substances, hydrolytic enzymes.’ These granules appear in the immature cell as large membrane bounded structures with a homogeneous electron dense content; as the cell matures, a crystalloid inclusion commonly develops within the granule.2.3 Electron mi...

Heterogeneous ribonucleoproteins (hnRNPs) have key roles in RNA biogenesis, including pre-mRNP assembly, transport and cytoplasmic localization. Here we show by biochemical fractionation of nuclear extracts and protein-protein interaction assays that the A/B-type hnRNP CBF-A is in a multiprotein complex with hnRNP A2 and A3 and hnRNP U. Using RNA affinity chromatography and gel retardation assa...

Eosinophils were separated from other types of cells in horse blood or rat peritoneal fluid by centrifugation in concentrated albumin solutions. Eosinophils did not appear to be damaged by this separation procedure. A technique was also devised for isolation of cytoplasmic granules from eosinophils, thus allowing studies on enzyme content of the granules. Granules from both horse and rat eosino...

Mast cells, neutrophils, basophils, natural killer and cytotoxic T cells are among the hematopoietic cell lines originating from immune cells. The importance of mammalian mast in innate immunity has piqued scientific community's interest. cytoplasmic granules Histamine, proteoglycans, proteins, cytokines examples compounds (mediators) that produce granules. When external stimuli received, these...

Protocols were developed to automate image analysis and to track the movement of thousands of vesicular compartments in live cells. Algorithms were used to discriminate among different types of movement (e.g. random, caged, and directed). We applied these tools to investigate the steady-state distribution and movement of lytic granules (LG) in live natural killer (NK) cells by high-speed 3-dime...

Cytoplasmic ;saccules', bordered by monolayer membranes, containing round, virus-like particles, with a diameter of about 200 A, are described in liver cells of 11 patients suffering from viral hepatitis. The differences between this pattern, glycogenic clumps of granules, polysomes, and cytoplasmic autolysis processes are discussed.