In vitro organogenesis of citrus was studied for the genotypes Citrus sinensis cv. ‘Natal’, C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP – 0.0; 0.5; 1.0; 1.5, or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. auranti...

Multiple shoot clumps or microclones were collected from two onion (Allium cepa L.) varieties for bulblet formation. MS medium supplemented with various concentrations of BAP, Kn, 2ip and NAA used. The height responded was observed 90% 80% in Taherpuri Indian variety, respectively on 2.0 mg l-1 BAP+0.5 NAA. best formation obtained containing 1.0 2ip. Finally, the rooted bulblets hardened establ...

Berginia ligulata is medicinally significant and endangered plant of the western Himalayan Region. This used to treat many diseases such as urinary problems heart problems. By using leaf explant, an efficient rapid micropropagation protocol was developed. In in-vitro propagation, MS medium supplemented with benzyl amino purine (BAP), 1-naphthalene acetic acid (NAA) indol-3-acetic (IAA) induce m...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation and environment disturbance. This study was conducted to provide propagation methods through in vitro germination explant cultivation. Germination stimulated up 66.00% on Murashige Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 1-napthaleneacetic acid (NAA), 30...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...