and summary sections of this thesis are available online: http://hdl.handle.net/2077/24861 Cover picture: Human mesangial cell stained with anti-perlecan (green) and DAPI (blue)

The recently developed methods of non radioactive in situ hybridization of chromosomes offer new aspects for chromosome analysis. Fluorescent labelling of hybridized chromosomes or chromosomal subregions allows to facilitate considerably the detection of specific chromosomal abnormalities. For many biomedical applications (e.g. biological dosimetry in the low dose range), a fast scoring for abe...

Abstract Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as distinctive ingredient, and popularly known “locoto” or “rocoto”. This taxon also an outstanding source of resistance to biotic abiotic stresses well other valuable fruit traits for breeding the worldwidely C. annuum related species. In this study, chromosome complement cv. (2n = 24) was deeply c...

Abstract Cell viability is one of the critical quality attributes (CQAs) for analyzing and characterizing cellular therapeutic products. The characterization cell essential to ensure quality, safety efficacy during gene therapy development. In this work, we characterized compared two commonly used dual-staining methods, acridine orange/propidium iodide (AO/PI) orange/4′,6-diamidino-2-phenylindo...

The preparation of a DAPI (4',6-diamidino-2-phenylindole) derivative is described. The resulting derivative retains the fluorogenic property upon binding to double-stranded DNA. Its ability for bioconjugation through amide linkage is demonstrated.

rho 0 HeLa cells entirely lacking mitochondrial DNA (mtDNA) and mitochondrial transfection techniques were used to examine intermitochondrial interactions between mitochondria with and without mtDNA, and also between those with wild-type (wt) and mutant-type mtDNA in living human cells. First, unambiguous evidence was obtained that the DNA-binding dyes ethidium bromide (EtBr) and 4',6-diamidino...

We used in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes concurrently with microscopic examinations and methane measurements to characterize the microbial community of an anaerobic hybrid reactor treating pentachlorophenol (PCP) with a mixture of fatty acids (propionic, butyric, acetic and lactic) and methanol. Archaeal cells detected with probe ARC915 preva...