The cellular reactions in tuberculosis form one of the striking features of the disease and it is important to note that they can be produced by the dead bacilli. In 1890, Maffucci (1) studied the effect of subcutaneous injections of dead organisms; he recorded the formation of abscesses and the subsequent death of the animals from marasmus. The next year, Koch (2), in the well-known article de...

A small molecule tetraazacyclododecane-1,4,7,10-tetraacetic acid (Gd‑DOTA)4‑TPP agent is used to label human mesenchymal stem cells (hMSCs) via electroporation (EP). The present study assessed the cytotoxicity of cell labeling, in addition to its effect on cell differentiation potential. There were no significant adverse effects on cell viability or differentiation induced by either EP or cellu...

background: human peripheral blood nk cells constitutively express cd56 and cd16 antigens. peripheral blood nk cells seem to be strongly related with decidual nk cells, and may reflect the decidual nk cell functional status. there are varied reports concerning the relationship between nk cell cytotoxicity in women with recurrent spontaneous abortion. objective: to study nk activity in women wit...

During cell death of human cultured leukocytes (Jurkat, HL-60, THP-1, U937) and freshly prepared leukocytes, we observed a greater than 100-fold increase in the affinity of apoptotic and necrotic cells for fluorescein isothiocyanate (FITC)-heparin in comparison with live cells. Binding of FITC-heparin was reversed in the presence of high ionic strength, unlabeled heparan sulfate, and heparin an...

Background: Mass cytometry (Cytometry by Time of Flight, CyTOF) allows single-cell characterization on the basis of specific metal-based cell markers. In addition, other metals in the mass range such as silver can be detected per cell. Bacteria are known to be sensible to silver and a protocol was developed to measure both the number of affected cells per population and the quantities of silver...