results there were 39 cases with lymph node metastasis in 59 malignant tumors, and 0 cases in 6 benign follicular type tumors. the staining scores by jt-95 of the 39 tumors with lymph node metastasis were 5+ in eight cases and 6+ in 31 cases. on the other hand, the scores of 20 malignant tumors without lymph node metastasis were < 4+ in all of the cases. in the co-cultured assay, numerous adhes...

BACKGROUND New bioartificial liver devices are needed to supplement the limited supply of organ donors available for patients with end-stage liver disease. Here, we report the results of a pilot study aimed at developing a humanized porcine liver by transplanting second trimester human fetal hepatocytes (Hfh) co-cultured with fetal stellate cells (Hfsc) into the decellularized matrix of a porci...

The retinal extracellular matrix (ECM) provides architectural support, adhesion and signal guidance that controls development. Decellularization of the ECM affords great potential to tissue engineering; however, how structural affects in vitro development, differentiation maturation ocular cells remains be elucidated. Here, mouse porcine retinas were decellularized protein profile analyzed. Ace...

glycoconjugates and their programmed changes during the course of development in the cell-surface as well as in the extracellular matrix, are known to affect cell differentiation, cellular interaction and other developmental phenomena during embryogenesis. the purpose of this study was to localize n-acetylgalactosamin as well as fucose-containing glycoconjugates in situ during thymus developmen...

Decellularization of xenogeneic hearts offers an acellular, naturally occurring, 3D scaffold that may aid in the development of an engineered human heart tissue. However, decellularization impacts the structural and mechanical properties of the extracellular matrix (ECM), which can strongly influence a cell response during recellularization. We hypothesized that multiphoton microscopy (MPM), co...

Background: Stem cell therapy has been developed as an effective treatment method for the heart failure. Also, extracellular matrix has shown the positive effects in stem cell differentiation and myocardial tissue organization. Cardiogel is a native cardiac extracellular matrix (ECM) derived from cardiac fibroblasts. In the present study the role of cardiogel is examin...

ARTICLES DISCUSSED: Kolel, A., et al. Controlled strain of 3D hydrogels under live microscopy imaging. Journal Visualized Experiments. (2020). Yang, F., Wang, Z., and S. Dual raster-scanning photoacoustic small-animal imager for vascular visualization. Tamburrini, R., Detergent-free decellularization the human pancreas soluble extracellular matrix (ECM) production. Koch, S.E., A multi-cue biore...

Roy, Sylvain, Paolo Silacci, and Nikolaos Stergiopulos. Biomechanical proprieties of decellularized porcine common carotid arteries. Am J Physiol Heart Circ Physiol 289: H1567–H1576, 2005. First published May 20, 2005; doi:10.1152/ajpheart.00564.2004.—To analyze the effects of decellularization on the biomechanical properties of porcine common carotid arteries, decellularization was performed b...

BACKGROUND Only limited information is available regarding the influence of decellularization on the extracellular matrix in heart valves. Within the extracellular matrix proteoglycans (PG) play a central role in the structural organization and physical functioning of valves and in their capability of settling with endothelial and interstitial cells partially myofibroblasts. We have therefore e...

To the Editor: We have read the review article by Vesely1 regarding heart valve tissue engineering with great attention. The Vesely article describes recent state-of-the-art approaches to the creation of an optimal heart valve prosthesis. We feel, however, that some additional information needs to be added, as the reader could be misled by some statements made in the Vesely article. The develop...