Chromaffin vesieles were first purified by differential and density gradient centrifugation in isotonie (Percoll) gradients. In subsequent suerose gradients p38jsynaptophysin exhibited the same distribution as established marker substances of chromaffin vesieles. Quantification of immunoblots revealed that 750 ng p38{synaptophysin per mg of protein were present in the chromaffin vesieles reeove...

Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose density gradient centrifugation time course showed that the beta-glucosidase wa...

Comparative studies of RNA synthesis and polyribosome metabolism in normal (RFL) and transformed (RFL-T) cells under serum starvation show that their growth is more or less dependent upon serum factor. By limiting serum in the medium, 80 to 90% of RNA or protein synthesis is inhibited in RFL cells, while in RFL-T cells macromolecular synthesis proceeds at one-third to one-fourth the rate of con...

In this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined. By the Percoll density gradient centrifugation, bovine RBCs infected with active forms of B. ovata merozoites were essentially collected from one of the layers. Moreover, this method made it possible to eli...