background: dental pulp stem cells can be used in regenerative endodontic therapy. the aim of this study was to introduce an efficient method for dental pulp stem cells isolation. materials and methods: in this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. dental pulp stem cells were isolated by the following three different methods: (1) digestion of ...

INTRODUCTION A critical step in biomaterial selection effort is the determination of material as well as the biological properties of the target tissue. Previously, the selection of biomaterials and carriers for dental pulp regeneration has been solely based on empirical experience. METHODS In this study, first, the linear viscoelastic material functions and compressive properties of miniatur...

BACKGROUND Dental pulp stem cells can be used in regenerative endodontic therapy. The aim of this study was to introduce an efficient method for dental pulp stem cells isolation. MATERIALS AND METHODS In this in-vitro study, 60 extracted human third molars were split and pulp tissue was extracted. Dental pulp stem cells were isolated by the following three different methods: (1) digestion of ...

Dental preparation sometimes causes transient congestion, edema, and necrosis of the pulp. We hypothesized that nitric oxide (NO) is involved in the pathophysiological changes in pulp after preparation. The mRNA and protein expression of the inducible isoform of NO synthase (iNOS) was examined in murine pulp after dental preparation. The effects of NO on the proliferation, mineralization, and a...

Capík I . , V. Ledeck ̆, A. ·evãík: Effects of Flumetazone on Exposed Dental Pulp of Dogs. Acta Vet. Brno 2003, 72: 279-283. Dental pulp metabolism plays very important role throughout the tooth life. Its role is essential particularly in the first two-three years. This is the time of the highest dentin deposition which provides sufficient firmness to withstand common everyday trauma. This study...

OBJECTIVE The aim of this study was to determine the prevalence of pulp stones (PS) in a Turkish dental patient population with respect to sexes and dental localization in relation between sex and this anomaly. MATERIALS METHODS A retrospective study was performed using bitewing radiographs of 814 patients ranging in age from 15 to 65. All data (age, sex, and location) was obtained from the f...

OBJECTIVES Polymerized dental resin materials release residual monomers that may interact with pulp tissues. We hypothesized that dental adhesives might cause cytotoxicity in pulp cells via the generation of reactive oxygen species (ROS), which may also contribute to genotoxic effects in vitro. METHODS For cytotoxicity testing, transformed human pulp-derived cells were exposed to extracts of ...

The viability of dental pulp depends largely on regional blood flow, as in other organs. Measurement of absolute pulpal blood flow (PBF) and comparisons with blood flow in other organs allow the prediction of microvascular regulation in dental pulp. In previous studies, PBF was measured in dogs mainly with radioactive microspheres. However, this established technique is inaccessible to many inv...

The main purpose of this study is to examine whether the stimulation of an exclusively pain-sensing receptive field (dental pulp) could determine cardiorespiratory effects in animals in which the cortical integration of the peripheral information is abolished by deep anesthesia. In 15 anesthetized (alpha-chloralose and urethan) rabbits, low (3-Hz)- and high-frequency (100-Hz) electrical dental ...

Neural stem cells (NSCs) have a high potency for differentiation to neurons and glial cells for replacement of damaged cells and paracrine effects for the regeneration and remyelination of host axons. Dental pulp is known to have a potential to differentiate into neural-like cells; therefore, dental pulp may be used as an autologous cell source for neural repair. In this study, we selectively e...