It has been shown (Bernheimer & Ruffier, 1951 ) that physical disruption of streptococci belonging to Lancefield groups A, B, C and D results in the liberation of a substance which partially inhibits the depolymerizing action of group A streptococcal deoxyribonuclease. One of the striking characteristics of the inhibitory substance is its specificity, for although it inhibits the deoxyribonucle...

Empyema is a severe complication of different diseases and traumas. Management of this complication is difficult and should comprise general and local procedures. The general procedure is mainly based on administering wide-spectrum antibiotics. Local management depends on patient general condition, but in all cases the essential procedure is to insert a drain into the pleural cavity and to evac...

Levine, James S. (Yale University, New Haven, Conn.), and Norman Strauss. Lag period characterizing the entry of transforming deoxyribonucleic acid into Bacillus subtilis. J. Bacteriol. 89:281-287. 1965.-The kinetics of appearance of transformants as a function of time of exposure to deoxyribonucleic acid (DNA) has been studied in Bacillus subtilis. A short lag period of approximately 1 min at ...

In cystic fibrosis (CF), large amounts of free leucocyte proteases are present in bronchial secretions, contributing to progressive lung damage. Recombinant, human deoxyribonuclease (rhDNase) is a new therapeutic agent that decreases sputum viscosity. However, deoxyribonuclease has been shown, in vitro, to release cationic enzymes from complexes with deoxyribonucleic acid (DNA). The present stu...

The relationship between heat-stable deoxyribonuclease and coagulase production was investigated in the interest of developing more rapid diagnostic and quantitative procedures for distinguishing toxigenic and pathogenic staphylococci from closely related saprophytic organisms.

Three enzymes with ribonuclease activity, one of which also had deoxyribonuclease activity, have been isolated and partially purified from corn seeds and seedlings. The purification of Ribonuclease I from mature seed was previously reported. This enzyme has a pH optimum near 5.0, is loosely adsorbed to carboxymethyl-cellulose, and has a molecular weight of 23,000, determined by gel filtration.R...

Respiratory insufficiency due to respiratory syncytial virus (RSV) bronchiolitis is partly due to the abundance of thickened mucus and the inability to clear it from the airways. Mucus in RSV bronchiolitis contains necrotic inflammatory and epithelial cells. The viscoelastic properties of purulent airway secretions are largely due to the presence of highly polymerized deoxyribonucleic acid (DNA...

We have identified a yeast protein that resembles actins from other eucaryotes in its tight binding to pancreatic deoxyribonuclease I, its copolymerizaton with purified muscle actin, its one-dimensional peptide map, and its apparent polymerization into 7-nm filaments. The yeast actin-like protein yielded a single spot on two-dimensional polyacrylamide gel electrophoresis, suggesting that a sing...

1. The activity of soluble ribonuclease and deoxyribonuclease first declined during senescence, but later increased during advanced stages of senescence. 2. Young leaves had very low ribonuclease or deoxyribonuclease activity associated with the chromatin, but the activity of these enzymes increased progressively during senescence until the leaves died. 3. No significant changes in the composit...

AIMS Immune system activation is a common feature of hypertension pathogenesis. However, the mechanisms that initiate this activation are not well understood. Innate immune system recognition and response to danger are becoming apparent in many cardiovascular diseases. Danger signals can arise from not only pathogens, but also damage-associated molecular patterns (DAMPs). Our first hypothesis w...