have been investigated as therapies to promote neuronal regeneration. Seeking to close in on driver mutations, Biankin and his team have generated more than 80 xenografts from the patient samples. They also are sequencing 40 cell lines created from the xenografts and are working with centers around the world to amass about 100 such cell lines. Beyond the axon-guidance pathway, Biankin estimates...

Real-time quantitative PCR (qPCR) is an indispensable molecular biology tool in the post-genome era. QPCR assays have become a robust and ideal tool for several applications such as clinical diagnostics, gene expression studies, biomarker discovery, and more. Using an intact genomic DNA (gDNA) template is crucial for generating meaningful qPCR data. Therefore, assessment of the gDNA integrity i...

A novel chromosomal translocation, t(2;ll)(q31;pl5), was identified in a patient with therapy-related acute myelogenous leukemia (I-AMI.). Fluorescence in \iin hybridization experiments mapped the breakpoint near NUP98; Southern blot analysis demonstrated that the nucleoporin gene NUP98 was disrupted by this translocation. We used rapid amplifi cation of cDNA ends to identify a chimi-rie iiiKN ...

Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of...

Modern communication and measurement system designs are increasing in complexity as the latest high-performance processors and DSPs enable new signal-processing techniques. As system requirements for speed and resolution increase, more capable Analog-to-Digital Converters (ADCs) emerge, and these in turn require higher-performance Analog Front Ends (AFEs). In many systems the AFE can be conside...

Using a well-defined in vitro model system for neoplastic progression, we have examined two basic characteristics in the acquisition of amplifi cation potential. Since Syrian hamster embryo fibroblasts can be trans formed by a variety of methods (spontaneously, chemically, virally, or by transfection with oncogenes). we determined whether the method of trans formation affects the capability of ...

A fluorescent, strongly Lewis-acidic borenium cation (Gutmann–Beckett acceptor number >100) was created using a tridentate formazanate ligand. This shows potential utility as colourimetric reactivity probe.

We estimate the effect of tensor parity nonconserving (PNC) interaction in 181TaO+ molecular cation. It can be used to probe unknown quadrupole distribution neutrons inside Ta nucleus. To this end, we evaluate constant which characterizes using relativistic Fock space coupled cluster theory for electronic structure modelling. The 3Δ1 state TaO+ cation measure PNC is found ground one agreement w...

Food allergens are proteins that are well tolerated by most, but can cause severe reactions in sensitive individuals. Since there is no cure for food allergy, strict adherence to an allergen-free diet is the only safe choice currently available for allergic consumers. Accurate food labeling can help consumers avoid foods containing an allergenic ingredient. Regulatory agencies have mandated the...

www.aacrjournals.org ABSTRACT Genomic characterization of recurrent breast and lung tumors developed over the course of 10 years in a 29-year-old patient with a germline TP53 mutation (LiFraumeni Syndrome) identifi ed oncogenic alterations in the HER2 and EGFR genes across all tumors, including HER2 amplifi cations, an EGFR -exon 20 insertion, and the fi rst-in-humans HER2 V659E mutation showin...