It is generally accepted that human immunodeficiency virus (HIV) is the etiologic agent of the acquired immunodeficiency syndrome and related diseases. In this report, we demonstrate the antiviral effect of nucleoside analogs 2',3'-didehydro-2',3'-dideoxythymidine (DHT) and 2',3'-didehydro-2',3'-dideoxycytidine (DHC) by using human T-cell lymphotropic virus type I-carrying MT-4 cells, which are...

We describe a highly sensitive method for the detection of 7-dehydrocholesterol (7-DHC), the biosynthetic precursor of cholesterol, based on its reactivity with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) in a Diels-Alder cycloaddition reaction. Samples of biological tissues and fluids with added deuterium-labeled internal standards were derivatized with PTAD and analyzed by LC-MS. This protocol...

OBJECTIVE Large middle cerebral artery stroke (space-occupying middle-cerebral-artery (MCA) infarction (SO-MCAi)) results in a very high incidence of death and severe disability. Decompressive hemicraniectomy (DHC) for SO-MCAi results in large reductions in mortality; the level of function in the survivors, and implications, remain controversial. To address the controversy, we pooled available ...

OBJECTIVE Our purpose was to determine the orthodontic treatment need in adolescent Kuwaitis, adjusting for treatment experience and acquired need due to mesial migration or loss of the first molars, and to assess the orthodontic manpower requirements. SUBJECTS AND METHODS A population-based sample of 753 boys and 728 girls, representing about 7% of all 13- to 14-year-old Kuwaitis, was examin...

Functional analysis of cytoplasmic dynein in Caenorhabditis elegans has revealed a wide range of cellular functions for this minus-end-directed motor protein. Dynein transports a variety of cargos to diverse cellular locations, and thus cargo selection and destination are likely regulated by accessory proteins. The microtubule-associated proteins LIS-1 and dynein interact, but the nature of thi...

Smith-Lemli-Opitz syndrome (SLOS) is a genetic disorder characterized by low plasma cholesterol and high 7-dehydrocholesterol (7-DHC). Synthesis of cholesterol and 7-DHC and its metabolites is regulated by HMG-CoA reductase, whose activity can be measured by 24-h excretion of its product mevalonate. We devised a simple, non-invasive method for collecting 24-h urine in our subjects. With a backg...

Vinegar and wine processing of medicinal plants are two traditional pharmaceutical techniques which have been used for thousands of years in China. Tetrahydropalmatine (THP), dehydrocorydaline (DHC) and protopine are three major bioactive molecules in Rhizoma Corydalis. In this study, a simple and reliable HPLC method was developed for simultaneous analysis of THP, DHC and protopine in rat tiss...

BackgroundSupporting young people's mental and physical health is a global policy priority, but detecting need facilitating access to services support challenging. We aimed explore the perspectives of people professional stakeholders acceptability, utility, effectiveness school-based online wellbeing screening tool, Digital Health Contact (DHC), which has linked follow-up support. The DHC was i...

There is a large potential to integrate substantial shares of renewable energy and waste heat sources in district heating and/or cooling networks (DHC), reducing dependency DHC on fossil fuels ultimately leading more efficient sustainable system. Several EU funded projects are currently working this topic. The objective the workshop aimed share WEDISTRICT project concept with other sister inter...