The free radical nitric oxide (NO) is known to play an important role in the biology of human cancers, including lung cancer. However, it is still not clear how elevated amounts of nitric oxide affect tumor development and propagation. Herein we develop an in vitro model system to study these effects in lung tumor cells. Two cell lines—one human lung adenocarcinoma (A549) and one mouse adenocar...

Magnetic chitosan nanoparticles, activated by glycidyl methacrylate, can be functionalized grafting diethylenetriamine (DETA) and dithizone for improving U(VI) sorption at pH around 5. The physicochemical properties of the materials have been characterized a wide variety analytical techniques. Uranyl increases with (progressive deprotonation amine sulfur groups). Uptake kinetics are controlled ...

The paper describes the chemical modification of PVC consisting in introduction amino groups (1,4-dioxane, diethylenetriamine), and then cross-linking with dichlorodiethyl ether. optimal extraction time was obtained for polymer that had more chlorine atoms substituted diethylenetriamine polymers were characterized by infrared spectroscopy (FTIR), elemental analysis (CHN) differential thermal (D...

We investigated the effect of a nitric oxide (NO)-releasing derivative of aspirin (NCX-4016) on a mitochondria-dependent model of apoptosis in human umbilical endothelial cells (HUVEC). Exposure of HUVEC to staurosporine caused a progressive fall in mitochondrial membrane potential (DeltaPsi(m)) and apoptosis. Exposure to an NO donor, (z)-1-[2-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium...

Myosin phosphatase target subunit 1 (MYPT1) is the regulatory subunit of myosin light chain phosphatase (MLCP). It plays a critical role in vasodilatation induced by cGMP-elevating agents such as nitric oxide (NO). The present study was performed to determine the role of MYPT1 in the development of tolerance of the pulmonary artery to NO. Incubation of isolated porcine pulmonary arteries for 24...

We investigated the mechanisms by which nitric oxide (NO) from an NO donor (DETA/NO) regulates proliferation of pheochromocytoma PC12 cells. The NO donor stimulated proliferation at low concentrations, but reversibly and completely inhibited proliferation at higher concentrations. The stimulation (but not the inhibition) of proliferation was apparently due to NO stimulation of soluble guanylate...

Let A,B,C be n× n positive semidefinite matrices. It is known that det(A+ B + C) + detC ≥ det(A+ C) + det(B + C), which includes det(A+B) ≥ detA+ detB as a special case. In this article, a relation between these two inequalities is proved, namely, det(A+ B + C) + detC − (det(A+ C) + det(B + C)) ≥ det(A+ B)− (detA+ detB).

diabetes is characterized by a nitric oxide deficiency at the wound site. diabetes is a factor that influences all stages of wound healing. in animals with acute experimental diabetes induced by streptozotocin (stz), the early inflammatory responses after wounding is impaired, fibroblast and endothelial cell proliferation is reduced as well as accumulation of reparative collagen and gain in wou...

A cultured porcine pulmonary artery (PA) model was used to examine the effects of prolonged nitric oxide (NO) treatment on the response to acutely applied NO, cGMP analog, or atrial natriuretic peptide (ANP). Twenty-four-hour treatment with the NO donor (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NO) resulted in >10-fold decrease in the response to acutely app...

Activation of a soluble guanylyl cyclase plays an important role in nitric oxide (NO)-induced vasodilation. Recently, we have reported that NO increases the calcium-activated potassium (K(Ca)) channel activity in vascular smooth muscle cells from coronary arteries. The present study examined the role of the soluble guanylyl cyclase in the control of basal activity of the K(Ca) channels and in m...