A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari, R. conori, and Coxiella burneti. Plaque-forming units (PFU) were compared to ...

Odorant diluents are generally chosen because of their odorless qualities, allowing them to dilute a target odorant without otherwise altering its perception. Unpublished observations from our laboratory, however, suggest that mineral oil (MO), a common diluent for oil-based odorants, may possess a distinct odor when used in the behavioral testing of mice. To test this, mice were trained to dis...

BACKGROUND Considerable, unexamined controversy exists surrounding the use of the highest vs the lowest FEV(1) for calculating the provocative concentration causing a 20% fall in FEV(1) (PC(20)) during direct bronchoprovocation challenges. OBJECTIVE To compare the PC(20) calculated using the lowest FEV(1) post-diluent and post-histamine/methacholine vs the PC(20) calculated using the highest ...

Nine different formulations were prepared from the prepolymer, 2,2-bis-[4-(2-hydroxy-3-acryloxy prop-1-oxy)phenyl] propane with various functional diluents namely, monofunctional diluent, Di(ethylene glycol)ethylether acrylate, difunctional diluent, tri(ethylene glycol) dimethacrylate and trifunctional diluent, trimethylolpropane ethoxylate (1-ethoxylate/hydroxyl) triacrylate in the presence of...

Relative rate techniques were used to study the kinetics of the reactions of Cl atoms and OH radicals with ethylene glycol diacetate, CH3C(O)O(CH2)2OC(O)CH3, in 700 Torr of N2/O2 diluent at 296 K. The rate constants measured were k(Cl + CH3C(O)O(CH2)2OC(O)CH3) = (5.7 +/- 1.1) x 10(-12) and k(OH + CH3C(O)O(CH2)2OC(O)CH3) = (2.36 +/- 0.34) x 10(-12) cm3 molecule-1 s-1. Product studies of the Cl a...

Although detection thresholds for odors are commonly measured in academic and medical settings, the influences of procedural factors on threshold values are poorly understood. The present study evaluated the influences of (i) trial sequence position and (ii) diluent type on the threshold value for the rose-like odorant phenyl ethyl alcohol. In Exp. 1, detection thresholds were measured in 24 su...

Prompt administration of intravenous (i.v.) dantrolene sodium (DS) is the primary determinant of successful treatment of malignant hyperthermia (MH) syndrome. Because DS has a long reconstitution time for use in treating an MH crisis, we evaluated an alternative technique for hastening the reconstitution. Simulating real-world conditions, with equipment common to the operating room environment,...

We report the case of a 31 year old woman who presented for management of her documented polycystic ovarian syndrome (PCOS). Her body mass index at the time of treatment was 35.9 kg/m 2. Previously, she had conceived following ovulation induction with urinary gonadotrophins in 1992 and gave birth to a son. She now wished to have another child. Induction of ovulation with clomiphene citrate (Clo...

Four experiments were conducted to study the following: i) the influence of different concentrations of sucrose (0.15, 0.3 and 0.5 M with osmolality of 308, 500 and 760 mOsm/kg, respectively) in diluents and control diluent (370 mOsm/kg) on intensity of motility and progressive motility of goat sperm without rehydration and freezing step in four incubation periods (0, 0.5, 2 and 4 h after dilut...