The aim of this study was to choose the optimal variant of PCR examination of faeces to detect Echinococcus multilocularis infection which would allow to reduce the influence of different inhibitors in faeces. The investigation was carried out by comparison of 3 different methods of DNA isolation from faeces and different DNA dilutions used in PCR. Thirty five intestines of red foxes were used....

the aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (casa). the semen samples were collected from three ghezel rams. samples were diluted in seminal plasma (sp), phosphate-buffered saline (pbs) containing 1% bovine serum albumin (bsa) and bioexcell. the motility parameters that computed and rec...

the present study was aimed to test the efficacy of adding vitamins c or e to tris-fructose-egg yolk diluent to increase awassi ram sperm storage period at 5 ˚c. semen samples from six mature awassi rams were used in this study. the semen samples were diluted by tris-glucose-egg yolk. diluted semen sample was divided into three parts. the first part was added with 0.9 mg ml-1 vitamin c, the sec...