In order to establish prerequisite conditions for hyphenation of inductively coupled plasma– atomic emission spectrometry with liquid chromatography, the effects on plasma signal by loading of low concentrated solutions of tartaric, citric and dipicolinic acid were studied. The intensities of Mn, Li, Mg, Cd and Cu lines were observed in axial and radial signal collection mode. Influences of dif...

Dipicolinic acid (DPA), present in large amount bacterial spores, has been proposed to act as an endogenous photosensitizer spore photoproduct formation. The mechanism involves a triplet-triplet energy transfer from DPA thymine. However, up now, no spectroscopic studies have performed determine the interaction between compound and nucleobase, probably due its photolability aqueous solutions. He...

Fluorescence emission and excitation spectra were measured over a 7-day period for Bacillus subtilis (Bs), a spore-forming, and Staphylococcus aureus (Sa), a nonspore-forming bacteria subjected to conditions of starvation. Initially, the Bs fluorescence was predominantly due to the amino acid tryptophan. Later, a fluorescence band with an emission peak at 410 nm and excitation peak at 345 m, fr...

Spectral changes of bovine serum albumin (BSA) in the presence of dipicolinic acid (DPA) were studied with UV/Vis absorption and fluorescence spectroscopy. The change in UV/vis spectra of BSA in the presence of DPA indicated the protein weakly interacting with DPA. The DPA quenching to the fluorescence of BSA leads to the calculation of the BAS-DPA association constant K of 8.9 x 104 Lmol-1 at ...

If robust spectroscopic techniques are to be developed for the detection and identification of pathogens, one must understand the relevant spectroscopic properties of the target molecules. In this paper, we employ density functional theory (DFT) to study the structural, electronic, vibrational, optical, and magnetic properties of dipicolinic acid (DPA) and its dianion DPA . Our full geometrical...

Klebsiella pneumoniae strains co-producing klebsiella pneumoniae carbapenemase (KPC) and verona integron-encoded metallo-beta-lactamase (VIM) are frequently isolated in Greece and have also occurred in other European countries. Conventional combined disc tests exhibit low sensitivity against these emerging pathogens. We have evaluated modifications of the KPC/Metallo-β-Lactamase Confirmation ki...

Raman spectra of dipicolinic acid (DPA) are important for detection of bacterial spores, since DPA and its salts present one of their major components. The implementation of a deeply cooled CCD camera in combination with pulsed excitation at 532 nm allowed measuring well-resolved Raman spectra of the DPA in different forms. Powder preparations, crystals grown from saturated solutions and aqueou...

Bacteria of Bacillus species sporulate upon starvation, and the resultant dormant spores germinate when the environment appears likely to allow the resumption of vegetative growth. Normally, the rates of germination of individual spores in populations are very heterogeneous, and the current work has investigated whether spore-to-spore communication enhances the synchronicity of germination. In ...

The title proton-transfer compound, C(3)H(12)N(2) (2+)·2C(7)H(4)NO(4) (-)·2C(7)H(5)NO(4)·8H(2)O or (pnH(2))(pydcH)(2).2(pydcH(2))·8H(2)O, was obtained by the reaction of pyridine-2,6-dicarboxylic acid (pydcH(2)) and propane-1,3-diamine (pn) in aqueous solution. Both neutral and monoanionic forms of the diacid are observed in the crystal structure. The negative charge of two monoanions is balanc...

Pressure-assisted thermal sterilization (PATS) is a new technology to inactivate bacterial spores and ensure food safety. Little has been known about the effects of PATS combining with ε-PL on spore’s nucleic acid, enzymes other key substances. This study aimed investigate inactivation effect B. subtilis. The were treated pressure 600 MPa at 25 °C, 65 °C 75 0.1% 0.3%. After treatment, survival ...