We describe a method for preparing purified erythroblastic nests in large numbers (approximately 10(6)/run) in three steps: (1) induction of splenic erythropoiesis in mice, (2) preparative differential centrifugation for the removal of erythrocytes and single cells from spleen cell suspensions, and (3) sedimentation in an isokinetic gradient of Ficoll 400 in Joklik's modification of minimum ess...

BACKGROUND Extracellular vesicles (EV), the collective term for vesicles released from cells, consist of vesicle species ranging in size from 30 nm to 5 µm in diameter. These vesicles are most commonly isolated by differential centrifugations, which pellets particles based on their differential movement through the liquid medium in which they are immersed. Multiple parameters, including the uti...

The isolation of 55 S particles from mitochondria has been reported. Evidence has been presented which emphasizes the importance of adequate control of contamination during preparation of mitochondria. A satisfactory mitochondrial purification method was attained through the use of radioactive microsomal fractions and electron microscopy to assess the contamination of mitochondria and ribosomes...

Twenty-nine types of mouse tumor and two normal mouse tissues were examined in the frozen state by electron spin resonance spectroscopy, and the redox states of some components were characterized. Intracellular organelles of mouse hepatoma and of normal mouse liver were separated by differential centrifugation and examined by elec tron spin resonance spectroscopy. The major difference between t...

The perspective-n-point (PnP) problem, which estimates 3D rotation and translation of a calibrated camera from n pairs of known 3D points and corresponding 2D image points, is a classical problem but still fundamental in the computer vision community. It is well studied that the PnP problem can be solved by at least three points [1]. If n ≥ 4, the PnP problem becomes a nonlinear problem where t...

This paper proposes a globally optimal direct least squares (DLS) method for the PnP problem with Cayley parameterization. First we derive a new optimality condition without Lagrange multipliers, which is independent of any rotation representations. Then, we show that the new equation can be solved by several types of parameterizations and among them, Cayley parameterization is the most efficie...

in this paper, differential transform method (dtm) is described and is applied to solve systems of nonlinear ordinary differential equations which is arising in hiv infections of cell. intervals of validity of the solution will be extended by using pade approximation. the results also will be compared with those results obtained by runge-kutta method. the technique is described and is illustrat...

MOTIVATION Massive amounts of genome-wide gene expression data have become available, motivating the development of computational approaches that leverage this information to predict gene function. Among successful approaches, supervised machine learning methods, such as Support Vector Machines (SVMs), have shown superior prediction accuracy. However, these methods lack the simple biological in...