نتایج جستجو برای: dna amplification

تعداد نتایج: 547367  

Journal: :Analytical Chemistry 2021

5-Hydroxymethylcytosine (5hmC) is a modified base present at low levels in various mammalian cells, and it plays essential roles gene expression, DNA demethylation, genomic reprogramming. Herein, we develop label-free template-free chemiluminescent biosensor for sensitive detection of 5hmC DNAs based on 5hmC-specific glucosylation, periodate (IO4+) oxidation, biotinylation, terminal deoxynucleo...

Journal: :Journal of the Japanese Association for Infectious Diseases 1992

Journal: :The Journal of Antibiotics 2003

Journal: :Nucleic Acids Research 1992

Journal: :Biotechnology and Genetic Engineering Reviews 1986

H. Hosseini M. Razzazian M.H. Bozorgmehrifard S.A. Pourbakhsh, S.M. Peighambari

  Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...

2006
Bruce E. Johnson Robert W. Makuch Alfreda D. Simmons Adi F. Gazdar David Burch Alan W. Cashell

Tumor specimens procured from 38 different small cell lung cancer patients were studied for DNA amplification of the myc family of protooncogenes (c-myc, \-myc, and 1.-myc). Six of the 38 specimens (16%) had 4-fold or greater myc family DNA amplification (N-myc in 4 and Lmyc in 2). All 6 tumors with amplification came from patients who had received combination chemotherapy. The myc family gene ...

2017
Michael Marotta Taku Onodera Jeffrey Johnson G. Thomas Budd Takaaki Watanabe Xiaojiang Cui Armando E. Giuliano Atsushi Niida Hisashi Tanaka

Oncogene amplification confers a growth advantage to tumor cells for clonal expansion. There are several, recurrently amplified oncogenes throughout the human genome. However, it remains unclear whether this recurrent amplification is solely a manifestation of increased fitness resulting from random amplification mechanisms, or if a genomic locus-specific amplification mechanism plays a role. H...

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