The signal transduction system acts either monodirectionally (synergistically) or bidirectionally (antagonistically) in different tissues. We activated the signal transduction system in BeWo human choriocarcinoma cells and studied the effects on human chorionic gonadotropin (hCG) secretion, cell proliferation, and DNA synthesis. Protein phosphorylation in the cytosolic fraction was also studied...

OBJECTIVE To evaluate the success of systemic single-dose methotrexate (MTX) treatment in patients with ectopic pregnancy (EP) and to investigate factors related to treatment success. METHODS This retrospective study had been performed in Yildirim Beyazit University between January 2010 and December 2015. Demographic and clinical characteristics, ultrasonografic findings, pretreatment serum β...

Objective To compare the results of expectant management, single and multidose methotrexate (MTX) and surgical management of ectopic pregnancy (EP). Materials and Methods In this retrospective cohort study, the original files of 233 patients who were treated for EP between May 2009 and December 2016 were analyzed. The patients were assigned to the following groups based on the applied treatme...

Received: Revised: Accepted: May 18, 2014 May 23, 2014 May 28, 2014 The study has been designed for the induced breeding of Giant Catfish, Sperata seenghala using hormonal analogues at Aquaculture and Fisheries Program, National Agricultural Research Center, Islamabad. The different hormonal analogues used in this study were ovaprim, HcG, LHRH and ovatide. The dose of ovaprim was 0.5 ml/kg 1b.w...

The effect of inhalation of hydrogen-containing gas (1.3% hydrogen + 20.8% oxygen + 77.9% nitrogen) (HCG) on radiation-induced dermatitis and on the healing of healing-impaired skin wounds in rats was examined using a rat model of radiation-induced skin injury. An X-ray dose of 20 Gy was irradiated onto the lower part of the back through two holes in a lead shield. Irradiation was performed bef...

Several clonal Leydig tumor cell lines have been established by adapting the transplantable Leydig tumor, M548OP, to culture. One of these cell line, MLTC-1, has been characterized with regard to the gonadotropin-responsive adenylate cyclase system. The binding of 125I-labeled human chorionic gonadotropin (hCG) was blocked by excess unlabeled hCG and lutropin (LH) but not by follitropin, thyrot...

The effect of inhibition of ornithine decarboxylase with difluoromethylornithine (DFMO) and the resultant lowering of polyamine levels upon human chorionic gonadotropin (hCG) production in JEG-3 choriocarcinoma cells was investigated. DFMO (10 mM) totally inhibited ornithine decarboxylase activity. In DFMO-treated cells, cellular spermidine concentrations fell to nondetectable levels (less than...

The present study was performed to optimize a superovulation protocol in rats in order to produce a large number of good-quality embryos suitable to develop rat embryonic stem (rES) cells. We first evaluated the ovulation kinetics of three rat strains: Wistar, Fisher and ACI/N. Animals (n=30 per strain) were treated with 50 IU of pregnant mare serum gonadotrophin (PMSG), and ovulation was induc...

A method for maintaining primary cultures of ovarian cells in serum-free medium in which the metabolic functions of the androgen-producing cells can be studied is described. Freshly dispersed cells from ovaries of hypophysectomized immature rats contained interstitial cells with specific, high affinity lZ5I-human chorionic gonadotropin (hCG) binding sites which were functionally coupled to aden...

In circumstances such as in vitro fertilization (IVF) or in patients with luteal phase defect, one needs to ensure the establishment of pregnancy as soon as possible. At the same time, a question arises: How can a physician make sure that the β-hCG is of an endogenous source and not the residue of the prescribed drugs (hCG)? In this article we have studied the elimination time of 1M inject...