Antibiotic G-418 is a new aminoglycoside produced as the major component by a new species of Micromonospora, M. rhodorangea NRRL 5326. The antibiotic is prepared by submerged fermentation in a soybean-dextrin medium. Antibiotic G-418 is adsorbed on a cationic-exchange resin and separated from other impurities by passing it down a Dowex (1 x 2) resin column. The antibiotic, which contains 2-deox...

The occurrence of isomeric dinucleotides in the digest of deoxyribonucleic acid (DNA) with pancreatic deoxyribonuclease (DNase I) has been recently reported by Sinsheimer (1). The isomers were of the type (d)pXpY or (d)pYpX,’ the system of abbreviations being used which was suggested by Smith and Markham (2), and extended by Heppel et al. (3) and Privat de Garilhe and Laskowski (4). Although su...

In present study the photocatalytic degradation of commercial azo dye Direct Red 5B (DR5B dye) has been carried out in presence of recently developed photocatalyst: methylene Blue immobilized resin Dowex-11, under visible light. The effect of process parameters such as variation of the dye concentration, pH, light intensity and amount of catalyst loading on the reaction rate has been studied .T...

With the finding that xylitol is an intermediate in the glucuronate-xylulose pathway (2, 3)) experiments were undertaken to determine whether this pentitol occurs in human urine. Instead of xylitol, the analyses led to the isolation of n-arabitol from pentosuric urine as well as nL-arabitol from normal urine (4). During the fractionation of urinary polyols on Dowex l-borate columns, eluates wer...

Discharging phosphate through wastewaters into waterways poses a danger to the natural environment due to the serious risks of eutrophication and health of aquatic organisms. However, this phosphate, if economically recovered, can partly overcome the anticipated future scarcity of phosphorus (P) resulting from exhaustion of natural phosphate rock reserves. An experiment was conducted to determi...

The determination of myo-inositol trisphosphate by an enzymatic fluorometric assay is presented. The method involves the acid extraction of water-soluble inositol polyphosphates followed by separation by anion-exchange chromatography. Samples are subsequently neutralized by passage over a Dowex Cl- resin and elution with lithium chloride. Samples are then desalted with ethanol. Following dephos...

Calf liver microsomal apocytochrome bs was hydrolyzed with chymotrypsin and the resulting peptides were resolved on a Dowex 1 column. The isolation and partial amino acid sequences of the 10 chymotryptic peptides are described. The total amino acid composition of these peptides is equal to the sum of the residues present in the tryptic peptides. On the basis of the data obtained for the chymotr...

New alpha-glucosidase inhibitors, CKD-711 and CKD-711a were produced from the fermentation broth of Streptomyces sp. CK-4416 which was isolated from a forest soil of Jeju Island, South Korea. CKD-711 and CKD-711a were purified by Dowex 50W-2X and Sephadex G-10 column chromatography. In in vitro studies, CKD-711 showed a potent inhibitory activity against a-glucosidase from mammalian, but less i...

An enzymatic procedure for the synthesis of adenosine 5'-O-(3-thiotriphosphate) (ATP-r-S) on a 5O-mmol scale from dihydroxyacetone, sodium thiophosphate, ADP, and phosphoenol pyruvate is described. The synthesis uses polyacrylamide gel immobilized glycerokinase coupled to a pymvate kinase catalyzed ATP cofactor regeneration system, and polyacrylamide gel immobilized triosephosphate isomerase, g...