background: dendritic cells (dcs) are the most potent stimulators of primary t cell responses and play a key role in immune reactions after stem cell transplantation. very little is known about the cord blood (cb) dendritic cells and their potential involvement in the low incidence and lower severity of acute graft-versus-host disease after cb transplantation.   objectives: the aim of this stud...

serotonin receptors are involved in pathophysiology of schizophrenia and may mediate other neurotransmitter effects. we investigated serotonin receptors gene expression in peripheral blood mononuclear cells (pbmc) of naïve schizophrenic patients, before and after treatment. also serotonin receptor gene expression was compared in two treatment groups including haloperidol and olanzapine. the pbm...

This investigation addressed the role of major histocompatibility complex-encoded class I molecules in the activation and proliferation of human lymphocytes. We studied the effect of antibodies specific for HLA-A and HLA-B locus gene products on mitogen-stimulated peripheral blood mononuclear cell (PBMC) subpopulations. Three individually derived, well-characterized anti-HLA class I monoclonal ...

Severe congenital neutropenia (SCN) is a disorder of myelopoiesis characterized by severe neutropenia or absence of blood neutrophils secondary to a maturational arrest at the level of promyelocytes. We examined peripheral blood mononuclear cells (PBMC) of SCN patients who demonstrated normalization of their blood neutrophil counts in a phase II clinical study with recombinant human granulocyte...

patients and methods in the present cross-sectional study, 109 patients with established hiv infection, who visited a governmental and referral center for hiv screening in tehran (tehran west health center (twhc)) between may 2014 and july 2015 were enrolled according to the convenience sample strategy. after peripheral blood collection, isolation of plasma and peripheral blood mononuclear cell...

We studied the effect of sulfurous water thermal therapy on the phenotype and the proliferative response of peripheral lymphoid cells from ten subjects affected by chronic upper respiratory disease and from six suffering from articular and periarticular disorders. Sulfurous water (S-H2O) therapy did not modify the phenotype and function of peripheral blood mononuclear cells (PBMC) nor did it mo...

background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...

We previously demonstrated that stimulation of bovine peripheral blood mononuclear cells (PBMCs) with staphylococcal enterotoxin C (SEC), led to an inversion of the CD4(+):CD8(+)T cell ratio and generation of an atypical CD8(+)T cell subpopulation expressing CD26. In the present study, we examined T cell apoptosis and proliferation profiles of PBMC subpopulations in cultures stimulated with SEC...

Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-1 receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuc...

Transfusion or transplantation of T lymphocytes into an allogeneic recipient can evoke potent immune responses including, in immunocompromised patients, graft-versus-host disease (GVHD). As our previous studies demonstrated attenuated immunorecognition of red blood cells covalently modified with methoxy(polyethylene glycol) (mPEG), we hypothesized that T-cell activation by foreign antigens migh...