Tumor tissue explants are an attractive tool for personalized predictive tests, however their use is compromised by subjective nature of morphological assessment drug response. We aimed to identify reporter genes, which rapidly change level expression in drug-sensitive but not drug-resistant tumors. Fresh tumor tissues were obtained from patients with non-small cell lung cancer (NSCLC) undergoi...

BACKGROUND Cancer stem cells (CSCs) are responsible for multi-drug resistance in tumors. CD133 is a known biomarker of CSCs. The aim of this study is to screen for drug-resistant differentially expressed genes in CD133+ and CD133- lung cancer cells and to identify novel lung tumor drug-resistant genes. METHODS Magnetic activated cell sorting was used to isolate CD133+ and CD133- cells from hu...

multidrug-resistant (mdr) and extensively drug-resistant (xdr) tuberculosis (tb) are increasing globally. treatment options for these patients are very limited. although treatment of these patients with standardized regimen is associated with high mortality and morbidity, rational usage of new drugs might be promising. in this study we will review epidemiology of xdr-tb and tdr-tb in iran and t...

PURPOSE The goal of this study was to determine whether distinct gene expression profiles are associated with intrinsic and/or acquired chemoresistance in epithelial ovarian carcinoma. EXPERIMENTAL DESIGN Gene expression profiles were generated from 21 primary chemosensitive tumors and 24 primary chemo-resistant tumors using cDNA-based microarrays. Gene expression profiles of both groups of p...

Glioblastoma (GBM) is a cancer comprised of morphologically, genetically, and phenotypically diverse cells. However, an understanding of the functional significance of intratumoral heterogeneity is lacking. We devised a method to isolate and functionally profile tumorigenic clones from patient glioblastoma samples. Individual clones demonstrated unique proliferation and differentiation abilitie...

The treatment of advanced ovarian cancer with taxol is hindered by the development of drug resistance. The cellular target for taxol is the microtubule that is stabilized by the drug. Taxol preferentially binds to the beta subunit of tubulin of which there are six distinct isotypes in mammalian cells. We have used highly specific oligonucleotides and polymerase chain reaction to analyze express...

Murine L1210 leukemia cells resistant to the antineoplastic agent L-phenylalanine mustard have a 1.52.0-fold elevation in their cellular GSH and GSSG content as compared to drug-sensitive cells. Cellular uptake of ~-[U-'~C]cystine and its incorporation into GSH of the resistant tumor are correspondingly elevated. Synthesis of y-glutamylcysteine, GSH, and GSSG is elevated 1.5-2.0-fold in cell-f...

Chemotherapy is often used for breast cancer treatment, but individual outcome varies widely. We hypothesized that tumor proteomic profiles obtained prior to chemotherapy may predict the individual tumor response to treatment. The goal of our study was to explore feasibility of using proteomic profiling to preselect patients for an effective chemotherapeutic regimen. Tumors from 52 patients wit...

The emergence of drug resistance to chemotherapeutic agents is a major cause of treatment failure in cancer therapy. Therefore, much effort has been aimed at circumventing or reversing this undesired effect. Recently, we found that tumor cell lines selected for their multidrug-resistant phenotype can also exhibit increased levels of TAP mRNA and MHC class I proteins. This raised the question of...

P-glycoprotein (P-gp) is the most studied ATP-binding cassette transporter, known to be able to cause multidrug resistance. The relevance of P-gp for clinical drug resistance remains controversial, however. Using our K14cre;Brca1F/F;p53F/F conditional mouse model of hereditary breast cancer, we previously found that modestly elevated expression levels of the mouse P-gp genes Mdr1a (Abcb1a) and/...