INTRODUCTION Duplex ultrasound facilitates bedside diagnosis and hence timely patient care. Its uptake has been hampered by training and accreditation issues. We have developed an assessment tool for Duplex arterial stenosis measurement for both simulator and patient based training. METHODS A novel assessment tool: duplex ultrasound assessment of technical skills was developed. A modified dup...

This article describes the sequence-specific isolation and purification of intact double-stranded DNA (dsDNA) by oligonucleotide/PNA-assisted affinity capture (OPAC). The OPAC assay is based on selective tagging of a DNA duplex by biotinylated oligodeoxyribonucleotide (ODN) through formation of a so-called PD-loop. The PD-loop is assembled with the aid of a pair of PNA “openers”, which allow se...

Unmanned aerial vehicle (UAV)-aided two-way relaying networks are designed, where a UAV is deployed to assist multiple pairs of users in their information exchange. There two basic approaches for the user pairs' exchange within single time slot via relay. The first approach based on full-duplex, all participants operate full-duplex mode transmit and receive signals simultaneously. However, tran...

Full-duplex Infrastructure Nodes: Achieving Long Range with Half-duplex Mobiles

In this paper using strongly duplexes we introduce a new class of (semi)hypergroups. The associated (semi)hypergroup from a strongly duplex is called duplex (semi)hypergroup. Two computer programs written in MATLAB show that the two groups Z2n and Zn × Z2 produce a strongly duplex and its associated hypergroup is a complementary feasible hypergroup.

Synthesis of a neomycin-Hoechst 33258 conjugate is reported. The conjugate combines the ligands known to selectively bind in the duplex and the triplex groove. The conjugate stabilizes DNA duplex over DNA triplex. The conjugate selectively stabilizes the DNA duplex (in the presence of salt), with as little as 2 muM of the ligand leading to a DeltaTm of 25 degrees C.

We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences between an initially formed "reference" duplex (usually a Watson-Crick duplex) and a related "test" ...

RecA protein will bind to a gapped duplex DNA molecule and promote a DNA strand exchange with a second homologous linear duplex. A double-strand break in the second duplex is efficiently bypassed in the course of these reactions. We demonstrate that the bypass of double-strand breaks is not explained by a mechanism involving homologous interactions between two duplex DNA molecules, but instead ...