A new method for the isolation of simian virus 40 nucleoprotein complexes from nuclei of lytically infected cells is described. The method is based on the addition of a thiol-specific reagent, 5'5'-dithiobis(2-nitrobenzoic acid), to lysis and extraction buffers. By inhibiting an uncoating activity during simian virus 40 extraction, 5'5'-dithiobis (2-nitrobenzoic acid) allows the use of efficien...

The effect of soil extraction procedures and/or sample pretreatment (drying, freezing of the soil sample) on the extractability of cadmium and lead was tested in a model experiment, with an employment of optical emission and atomic absorption spectrometry methods. In the first part, 6 extraction procedures were compared: 2 mol l HNO3, 0.43 mol l CH3COOH, 0.05 mol l EDTA, Mehlich III extraction ...

In the present study shacking and incubation experiments were carried out in order to evaluate the changes in heavy metal solubility in the studied soils by addition of different concentration of Ethylene dinitrilo tetra acetic acid (EDTA), Diethylene triamine penta acetic acid (DTPA), and Nitrilo tri acetic acid (NTA). The effects of EDTA, DTPA and NTA application on solubility of Copper (Cu),...

Considerable interest has been focussed on the role of myosin light chain LC2 in the contraction of vertebrate striated muscle. A study was undertaken to further our investigations (Moss, R. L., G. G. Giulian, and M. L. Greaser, 1981, J. Biol. Chem., 257:8588-8591) of the effects of LC2 removal upon contraction in skinned fibers from rabbit psoas muscles. Isometric tension and maximum velocity ...

Aflatoxin B1 (AFB1) is a hepatocarcinogen produced by certain Aspergillus species growing on crops. After biotransformation in the liver, AFB1 generates several metabolites, one of which is AFB1 bound to lysine on serum albumin. AFB1-lysine (AFB1-lys) is a digest product of AFB1-albumin and is considered a biomarker of exposure to AFB1 in humans and animals. The objectives of this paper were to...

Electrokinetic soil remediation by electroosmosis and ionic migration is restricted to soluble substances. At a higher pH, the soil particles sorb more heavy metals than at a lower pH and the precipitation reactions of heavy metals are promoted. Both mechanisms make heavy metals immobile, rendering cleanup more difficult and electrokinetic extraction inefficient. In this study, the effectivenes...

Since DNA fingerprinting has become widely used ' 2 3 kb in genetic analysis of different species the original method (1) has undergone modifications, e.g. in DNA extraction, Southern transfer and labeling of the DNA probe. While applying this technique for pedigree monitoring of mouse inbred strains, it was important to isolate DNA from small blood samples in order to leave the animals alive. ...

In this study, we improved the eggshell-membrane separation process by separating the shell and membrane with EDTA solution, evaluating effects of three different extraction solutions (acetic acid, EDTA, and phosphate solution), and co-purifying multiple eggshell proteins with two successive ion-exchange chromatography procedures (CM Sepharose Fast Flow and DEAE Sepharose Fast Flow). The recove...